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B8EFC7

- SYI_SHEB2

UniProt

B8EFC7 - SYI_SHEB2

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Sbal223_3235
Organism
Shewanella baltica (strain OS223)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylate By similarity
Binding sitei608 – 6081ATP By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSBAL407976:GJ6Y-3339-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Sbal223_3235
OrganismiShewanella baltica (strain OS223)
Taxonomic identifieri407976 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000002507: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189194Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi407976.Sbal223_3235.

Structurei

3D structure databases

ProteinModelPortaliB8EFC7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8EFC7-1 [UniParc]FASTAAdd to Basket

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MSDYKFTLNL PETEFPMRGN LANREPEMLE RWTKDGLYQQ IRDSRIGRTP    50
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKTMSGFDAP YVPGWDCHGL 100
PIELKVEQKV GKPGQKISAA EFREECRKYA AEQVNGQRED FIRLGVLGDW 150
QNPYLTMDFS TEANIVRSLS KVIESGHLHK GVKPVHWCTD CGSALAEAEV 200
EYEDKTSPAI DVAFVAADSK AVAAKFGVSD YSHPVSMVIW TTTPWTLPAN 250
RALSLSPELD YSLVEFEKDG VTQALILAEV LVESCLTRYN VESHTVLGSA 300
KGAAFELVRF NHPFLDFDVP AILGDHVTTD AGTGIVHTAP GHGQDDFVVG 350
QKYGLEVANP VGDNGVYKPD TEYFAGQHVF KANDNVVALL REKSALLNHV 400
AYRHSYPHCW RHKTPIIFRA TPQWFISMDN HGLRTQALKE IEQTQWIPDW 450
GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VNRETEELHP DSVSLMERVA 500
SRIEQQGIQA WWDLDAAELL GDEAEQYRKV TDTLDVWFDS GSTFSSVVAA 550
RPEFHGHGVD LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD 600
GKGRKMSKSI GNVIAPQTVT NKLGADILRL WVAATDYSGE MTVSDEILNR 650
SADAYRRIRN TARFLLANLN GFEPAKDLVA VEDMVALDRW VVRRAAALQQ 700
EIIEAYEQYN FHIVTQKLMQ FCSVELGSFY LDIIKDRQYT AKQEGHARRS 750
CQSALYLISE AMVRWIAPIL SFTADEVWQL LPGERDAYVF TQEWYQGLKS 800
VTLATDLSDD YWQQLLTVRN EVNKVIEQAR RDKRIGGSLE AEVTLFADAA 850
LTEQLTHIGD ELRFVLLTSE AKVLPLADAT SEAVETELAS LKLLVASSTA 900
EKCERCWHHR EEVGTIEAHP TLCTRCVTNI EGDGEVRLFA 940
Length:940
Mass (Da):105,762
Last modified:March 3, 2009 - v1
Checksum:iF16316B4727B8ECA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001252 Genomic DNA. Translation: ACK47719.1.
RefSeqiYP_002359142.1. NC_011663.1.

Genome annotation databases

EnsemblBacteriaiACK47719; ACK47719; Sbal223_3235.
GeneIDi7085848.
KEGGisbp:Sbal223_3235.
PATRICi23482835. VBISheBal125792_3345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001252 Genomic DNA. Translation: ACK47719.1 .
RefSeqi YP_002359142.1. NC_011663.1.

3D structure databases

ProteinModelPortali B8EFC7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 407976.Sbal223_3235.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK47719 ; ACK47719 ; Sbal223_3235 .
GeneIDi 7085848.
KEGGi sbp:Sbal223_3235.
PATRICi 23482835. VBISheBal125792_3345.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SBAL407976:GJ6Y-3339-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OS223.

Entry informationi

Entry nameiSYI_SHEB2
AccessioniPrimary (citable) accession number: B8EFC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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