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B8EFC7

- SYI_SHEB2

UniProt

B8EFC7 - SYI_SHEB2

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella baltica (strain OS223)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
    Binding sitei608 – 6081ATPUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSBAL407976:GJ6Y-3339-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Sbal223_3235
    OrganismiShewanella baltica (strain OS223)
    Taxonomic identifieri407976 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000002507: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000189194Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi407976.Sbal223_3235.

    Structurei

    3D structure databases

    ProteinModelPortaliB8EFC7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8EFC7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKFTLNL PETEFPMRGN LANREPEMLE RWTKDGLYQQ IRDSRIGRTP    50
    FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKTMSGFDAP YVPGWDCHGL 100
    PIELKVEQKV GKPGQKISAA EFREECRKYA AEQVNGQRED FIRLGVLGDW 150
    QNPYLTMDFS TEANIVRSLS KVIESGHLHK GVKPVHWCTD CGSALAEAEV 200
    EYEDKTSPAI DVAFVAADSK AVAAKFGVSD YSHPVSMVIW TTTPWTLPAN 250
    RALSLSPELD YSLVEFEKDG VTQALILAEV LVESCLTRYN VESHTVLGSA 300
    KGAAFELVRF NHPFLDFDVP AILGDHVTTD AGTGIVHTAP GHGQDDFVVG 350
    QKYGLEVANP VGDNGVYKPD TEYFAGQHVF KANDNVVALL REKSALLNHV 400
    AYRHSYPHCW RHKTPIIFRA TPQWFISMDN HGLRTQALKE IEQTQWIPDW 450
    GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VNRETEELHP DSVSLMERVA 500
    SRIEQQGIQA WWDLDAAELL GDEAEQYRKV TDTLDVWFDS GSTFSSVVAA 550
    RPEFHGHGVD LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD 600
    GKGRKMSKSI GNVIAPQTVT NKLGADILRL WVAATDYSGE MTVSDEILNR 650
    SADAYRRIRN TARFLLANLN GFEPAKDLVA VEDMVALDRW VVRRAAALQQ 700
    EIIEAYEQYN FHIVTQKLMQ FCSVELGSFY LDIIKDRQYT AKQEGHARRS 750
    CQSALYLISE AMVRWIAPIL SFTADEVWQL LPGERDAYVF TQEWYQGLKS 800
    VTLATDLSDD YWQQLLTVRN EVNKVIEQAR RDKRIGGSLE AEVTLFADAA 850
    LTEQLTHIGD ELRFVLLTSE AKVLPLADAT SEAVETELAS LKLLVASSTA 900
    EKCERCWHHR EEVGTIEAHP TLCTRCVTNI EGDGEVRLFA 940
    Length:940
    Mass (Da):105,762
    Last modified:March 3, 2009 - v1
    Checksum:iF16316B4727B8ECA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001252 Genomic DNA. Translation: ACK47719.1.
    RefSeqiYP_002359142.1. NC_011663.1.

    Genome annotation databases

    EnsemblBacteriaiACK47719; ACK47719; Sbal223_3235.
    GeneIDi7085848.
    KEGGisbp:Sbal223_3235.
    PATRICi23482835. VBISheBal125792_3345.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001252 Genomic DNA. Translation: ACK47719.1 .
    RefSeqi YP_002359142.1. NC_011663.1.

    3D structure databases

    ProteinModelPortali B8EFC7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 407976.Sbal223_3235.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK47719 ; ACK47719 ; Sbal223_3235 .
    GeneIDi 7085848.
    KEGGi sbp:Sbal223_3235.
    PATRICi 23482835. VBISheBal125792_3345.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SBAL407976:GJ6Y-3339-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: OS223.

    Entry informationi

    Entry nameiSYI_SHEB2
    AccessioniPrimary (citable) accession number: B8EFC7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3