ID   FTHS_SHEB2              Reviewed;         580 AA.
AC   B8ECE1;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   27-MAR-2024, entry version 68.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Sbal223_3748;
OS   Shewanella baltica (strain OS223).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=407976;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OS223;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Chertkov O., Meincke L., Brettin T.,
RA   Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Ovchinnikova G., Brettar I., Rodrigues J., Konstantinidis K.,
RA   Tiedje J.;
RT   "Complete sequence of chromosome of Shewanella baltica OS223.";
RL   Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP001252; ACK48226.1; -; Genomic_DNA.
DR   RefSeq; WP_012588639.1; NC_011663.1.
DR   AlphaFoldDB; B8ECE1; -.
DR   SMR; B8ECE1; -.
DR   KEGG; sbp:Sbal223_3748; -.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000002507; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..580
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000185262"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   580 AA;  60863 MW;  7A60C1B3357091C9 CRC64;
     MLTDMDISSR ASLKNITELG ADLGLLPEEM MLFGHTKAKV ELSVLQRLAG QRKGKLIIVT
     AVTPTPHGEG KTVTSIGLTQ SLNAIGQKAC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
     EMNLHLTGDI HAVSSAHNLG AAAIAARLFH EARLGKTEFE AQSGQAFLDI APNEIRWHRV
     VDHNDRCLRQ IHVGLGDNNG PEYGSSFDIT AASELMAILA LSHDLADMRA RIGRLVLALN
     TQGQVITAED LGVAGAMTAI MADAIKPTLM QTLNGSPCLI HSGPFANIAH GNSSIIADDI
     ALRLADFVVT EGGFGSDMGF EKFCNIKVRQ SGQAPAAAVL VTTLKALKAN SGLATEVDSN
     APNIHVPNIN APDQARLEAG FANLNWHINN VARYGIPVVV GINRFATDSD AELQWLMEAV
     NASAAFGCEI SDAFSQGEAG AIALARTVVR AAETESQFKL LYPDEASLEA KLSTLAEVGY
     GASGVSLSIE AKQQAQQLTA LGYGHLPLCM AKTPLSISHD PSLKGVPKDF VVPVRELVLH
     AGAGFITALV GNVMTMPGLG LKPGYLKIDI DAKGEIVGLG
//