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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Shewanella baltica (strain OS223)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei416 – 4161NucleophileUniRule annotation
Active sitei469 – 4691Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciSBAL407976:GJ6Y-3130-MONOMER.
UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Sbal223_3028
OrganismiShewanella baltica (strain OS223)
Taxonomic identifieri407976 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000002507: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7437431,4-alpha-glucan branching enzyme GlgBPRO_1000147761Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi407976.Sbal223_3028.

Structurei

3D structure databases

ProteinModelPortaliB8EAX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

B8EAX1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MMTQANAYFY DGADVALLNG QYTDVFSLLG MHSANEGKAL IVRCFLRNAL
60 70 80 90 100
SVDVISIKDG RKVASLDKVN EQGLFAGTLG RRVKPFLYLL RVEYPQCQLD
110 120 130 140 150
IVDPYQFDSL LNSDDIYLFG EGSAERAYEF LGANWRQTQG VEGVHFCVWA
160 170 180 190 200
PNAKRVSVVG DFNHWDDTRH VMRQHLANGL WELFLPNVVE GAHYKFDLVY
210 220 230 240 250
QNGERHAKSD PMATQMECAP HNASIVPPKA HHSWNDTAWM SKRAATAWHK
260 270 280 290 300
APMSAYEVHL GSWRRKGEQG EQYLDYQDLI EQLIPYVKEQ GFTHIELMPI
310 320 330 340 350
SEFPFDGSWG YQPVGLYAPT HRFGDANGLK AFVDACHQAG IGIILDWVSA
360 370 380 390 400
HFPKDPHGLV RFDGTCLYEH EDPRKGTHPD WDTLIYNYDR GEVRSFLLSN
410 420 430 440 450
ACYWLREFHF DGLRLDAVSS MLYLDYSREP GQWLPNTYGG RENLEAINFL
460 470 480 490 500
QILNQRLYQA FPGICMIAEE STAFAGVTKP TDQQGLGFGF KWNMGWMNDS
510 520 530 540 550
LSYLGRDPLY RQFHHHQLTF SLMYAYTEQF MLSVSHDEVV HGKGSLLHKI
560 570 580 590 600
PGDDWQKFAT LRAYYGFMWG HPGKKLLFMG CEFGQRNEWN HNQSLDWHLL
610 620 630 640 650
AYEPHQGVQR WLKDLNHLYQ AMPALSVQDY EGAGFSWLDC ENSRDSIFTF
660 670 680 690 700
VRYGLAGDAP LVFVINMTPQ LHTGFRIGLP LAGDYREYLN SDSQIYGGSN
710 720 730 740
QGNAGTVVAE SLPWQGMAQS ALITVPPLGC LVIGPATGLA EAN
Length:743
Mass (Da):84,350
Last modified:March 3, 2009 - v1
Checksum:i75E4854CBE07E769
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001252 Genomic DNA. Translation: ACK47513.1.
RefSeqiWP_012588209.1. NC_011663.1.
YP_002358936.1. NC_011663.1.

Genome annotation databases

EnsemblBacteriaiACK47513; ACK47513; Sbal223_3028.
GeneIDi7088937.
KEGGisbp:Sbal223_3028.
PATRICi23482389. VBISheBal125792_3123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001252 Genomic DNA. Translation: ACK47513.1.
RefSeqiWP_012588209.1. NC_011663.1.
YP_002358936.1. NC_011663.1.

3D structure databases

ProteinModelPortaliB8EAX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi407976.Sbal223_3028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK47513; ACK47513; Sbal223_3028.
GeneIDi7088937.
KEGGisbp:Sbal223_3028.
PATRICi23482389. VBISheBal125792_3123.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciSBAL407976:GJ6Y-3130-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OS223.

Entry informationi

Entry nameiGLGB_SHEB2
AccessioniPrimary (citable) accession number: B8EAX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 4, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.