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B8EAX1 (GLGB_SHEB2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Sbal223_3028
OrganismShewanella baltica (strain OS223) [Complete proteome] [HAMAP]
Taxonomic identifier407976 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length743 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7437431,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000147761

Sites

Active site4161Nucleophile By similarity
Active site4691Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
B8EAX1 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 75E4854CBE07E769

FASTA74384,350
        10         20         30         40         50         60 
MMTQANAYFY DGADVALLNG QYTDVFSLLG MHSANEGKAL IVRCFLRNAL SVDVISIKDG 

        70         80         90        100        110        120 
RKVASLDKVN EQGLFAGTLG RRVKPFLYLL RVEYPQCQLD IVDPYQFDSL LNSDDIYLFG 

       130        140        150        160        170        180 
EGSAERAYEF LGANWRQTQG VEGVHFCVWA PNAKRVSVVG DFNHWDDTRH VMRQHLANGL 

       190        200        210        220        230        240 
WELFLPNVVE GAHYKFDLVY QNGERHAKSD PMATQMECAP HNASIVPPKA HHSWNDTAWM 

       250        260        270        280        290        300 
SKRAATAWHK APMSAYEVHL GSWRRKGEQG EQYLDYQDLI EQLIPYVKEQ GFTHIELMPI 

       310        320        330        340        350        360 
SEFPFDGSWG YQPVGLYAPT HRFGDANGLK AFVDACHQAG IGIILDWVSA HFPKDPHGLV 

       370        380        390        400        410        420 
RFDGTCLYEH EDPRKGTHPD WDTLIYNYDR GEVRSFLLSN ACYWLREFHF DGLRLDAVSS 

       430        440        450        460        470        480 
MLYLDYSREP GQWLPNTYGG RENLEAINFL QILNQRLYQA FPGICMIAEE STAFAGVTKP 

       490        500        510        520        530        540 
TDQQGLGFGF KWNMGWMNDS LSYLGRDPLY RQFHHHQLTF SLMYAYTEQF MLSVSHDEVV 

       550        560        570        580        590        600 
HGKGSLLHKI PGDDWQKFAT LRAYYGFMWG HPGKKLLFMG CEFGQRNEWN HNQSLDWHLL 

       610        620        630        640        650        660 
AYEPHQGVQR WLKDLNHLYQ AMPALSVQDY EGAGFSWLDC ENSRDSIFTF VRYGLAGDAP 

       670        680        690        700        710        720 
LVFVINMTPQ LHTGFRIGLP LAGDYREYLN SDSQIYGGSN QGNAGTVVAE SLPWQGMAQS 

       730        740 
ALITVPPLGC LVIGPATGLA EAN 

« Hide

References

[1]"Complete sequence of chromosome of Shewanella baltica OS223."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Ovchinnikova G., Brettar I., Rodrigues J., Konstantinidis K., Tiedje J.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS223.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001252 Genomic DNA. Translation: ACK47513.1.
RefSeqYP_002358936.1. NC_011663.1.

3D structure databases

ProteinModelPortalB8EAX1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING407976.Sbal223_3028.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK47513; ACK47513; Sbal223_3028.
GeneID7088937.
KEGGsbp:Sbal223_3028.
PATRIC23482389. VBISheBal125792_3123.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycSBAL407976:GJ6Y-3130-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_SHEB2
AccessionPrimary (citable) accession number: B8EAX1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries