B8E5A2 (GLO2_SHEB2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Sbal223_2327

Organism

Shewanella baltica (strain OS223) [Complete proteome] [HAMAP]

Taxonomic identifier

407976 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000184184
Sites
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	57	1	Zinc 1 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	60	1	Zinc 2 By similarity
	Metal binding	117	1	Zinc 1 By similarity
	Metal binding	134	1	Zinc 1 By similarity
	Metal binding	134	1	Zinc 2 By similarity
	Metal binding	172	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8E5A2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 4515D45104543D7A	FASTA	263	29,202
	10 20 30 40 50 60 MLTITAIKAF NDNYIWVLQQ QPHTQVYVVD PGDASVVIDY LEANQLTLAG ILLTHHHNDH 70 80 90 100 110 120 TGGVAELQAY SQDRLTVYGP DNEKIEGITH PLNATAQPRL TLDYMSGELQ VLDVPGHTAG 130 140 150 160 170 180 HIAYVIADAL FCGDTLFSGG CGRLFEGTPA QMLNSLQQLA QLPADTRVYC AHEYTFSNLK 190 200 210 220 230 240 FALAVNPNNR ALQDYNERAI ALRRQDKATI PSTIALERAI NPFLRASDTE IVDSIKQHFS 250 260 DLNHANLDEL GGFTLLRQWK DNF

References

[1]

"Complete sequence of chromosome of Shewanella baltica OS223." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Meincke L., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L. , Kyrpides N., Ovchinnikova G., Brettar I., Rodrigues J., Konstantinidis K., Tiedje J. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OS223.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001252 Genomic DNA. Translation: ACK46826.1.
RefSeq	YP_002358249.1. NC_011663.1.
3D structure databases
ProteinModelPortal	B8E5A2.
ModBase	Search...
Protein-protein interaction databases
STRING	B8E5A2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7087741.
GenomeReviews	Gene locus Sbal223_2327 in contig CP001252_GR.
KEGG	sbp:Sbal223_2327.
PATRIC	23480937. VBISheBal125792_2410.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	IGCGRVF.
ProtClustDB	CLSK906771.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_SHEB2

Accession

Primary (citable) accession number: B8E5A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program