B8E2N5 (TGT_DICTD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29

Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase

Gene names

Name:	tgt
Ordered Locus Names:	Dtur_1606

Organism

Dictyoglomus turgidum (strain Z-1310 / DSM 6724) [Reference proteome] [HAMAP]

Taxonomic identifier

515635 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Dictyoglomi › Dictyoglomia › Dictyoglomales › Dictyoglomaceae › Dictyoglomus › Dictyoglomus turgidum

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168
Catalytic activity	Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168		PRO_1000197999
Sites
	Active site	91	1	Nucleophile By similarity
	Metal binding	305	1	Zinc By similarity
	Metal binding	307	1	Zinc By similarity
	Metal binding	310	1	Zinc By similarity
	Metal binding	336	1	Zinc By similarity
	Binding site	92	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8E2N5 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 00D0043B5E99221C	FASTA	388	45,138
	10 20 30 40 50 60 MIFEIIVRDK KTRARLGKLK TFHGEINTPV FMPVGTQGAV KTLSPEEVEK VGAEIILSNT 70 80 90 100 110 120 YHLFLRPGHE IVRKGGGLHK FMGWEKPILT DSGGYQVFSL ARLRRIDEDG IYFNSHIDGT 130 140 150 160 170 180 RYFYTPELVM EIQKSLGSDI IMPLDICLGY GASYWETKEA LEITLRWLKR SIDYKNNSNM 190 200 210 220 230 240 DHQLLFGIVQ GGFYKELRKE AVERMLNIDL PGLALGGISV GEPKDKMYEI IDYTVSLLPE 250 260 270 280 290 300 EKPRYLMGVG APEDLVVGVS MGIDMFDCVL PTRLARHGVF YTSKGRKNIK NAQYKEDFSP 310 320 330 340 350 360 LEEDCDCYTC RKFTKAYIRH LFLQHETFSY RLLTIHNLRF LFRLMENIRK SIREGRLEEF 370 380 KKEFLTEYLR DDRENRLEKE ELWSKLLI

References

[1]

"Complete sequence of Dictyoglomus turgidum DSM 6724." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Mead D. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z-1310 / DSM 6724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001251 Genomic DNA. Translation: ACK42879.1.
RefSeq	YP_002353493.1. NC_011661.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	515635.Dtur_1606.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACK42879; ACK42879; Dtur_1606.
GeneID	7082124.
KEGG	dtu:Dtur_1606.
PATRIC	21812018. VBIDicTur93964_1655.
Phylogenomic databases
eggNOG	COG0343.
HOGENOM	HOG000223473.
KO	K00773.
OMA	MSPERSI.
ProtClustDB	CLSK2409568.
Enzyme and pathway databases
BioCyc	DTUR515635:GH4F-1656-MONOMER.
UniPathway	UPA00392.
Family and domain databases
Gene3D	3.20.20.105. 1 hit.
HAMAP	MF_00168. Q_tRNA_Tgt.
InterPro	IPR004803. Queuine_tRNA-ribosylTrfase. IPR002616. tRNA_ribo_trans. [Graphical view]
PANTHER	PTHR11962. PTHR11962. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
SUPFAM	SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_DICTD

Accession

Primary (citable) accession number: B8E2N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program