B8E2F6 (B8E2F6_DICTD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Dtur_1526

Organism

Dictyoglomus turgidum (strain Z-1310 / DSM 6724)

Taxonomic identifier

515635 [NCBI]

Taxonomic lineage

Bacteria › Dictyoglomi › Dictyoglomales › Dictyoglomaceae › Dictyoglomus

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B8E2F6 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: CF0D08AC19FE8488	FASTA	696	80,945
	10 20 30 40 50 60 MDNEKLIIVE SPAKAKTIEK ILGKEFKVLA SYGHIRDLPE KELGVDIENN FKPKYVLLPS 70 80 90 100 110 120 RKKIIDTIKD LSKKAEKLYL ATDPDREGEA IAWHLVEELK LPKEKFTRIE FHEITPRAIL 130 140 150 160 170 180 EAIKNPREID LDRVEAQQAR RILDRLVGYE LSPLLWEKVR KGLSAGRVQS VALRFICERE 190 200 210 220 230 240 EEIEKFIPEE YWYIYGEFRD EEKVLYGKLI LYKDEEVNVK NEQEVNKIIR ILNCLDYRID 250 260 270 280 290 300 KVVIKEEKRT PPPPFITSTL QQEASKRFGF PVGKTMKIAQ SLYEGVDIGN ERIGLITYMR 310 320 330 340 350 360 TDSTRIAPEA REEAVQYIES GFGKEYVGKE HKEKKKLGVQ DAHEAIRPTS VFRDPEKIRK 370 380 390 400 410 420 YLTDEQFKIY KLIWERFIAS QMNHAIFEVV EIYIKGGDYV FLSKGKKLKF AGFMSLYMEK 430 440 450 460 470 480 EENGEEKDEV NIDYVPEEGK NVKLTTLIPR QFFTEPPPRY TEATLVRTLE KYGVGRPSTY 490 500 510 520 530 540 ATIIETLKER KYVEVKDRTL VPTTLGKVVN DFLTKQFPHL IDVKFTARME EDLDKIESGK 550 560 570 580 590 600 VKAIEVLKKF YPAFKEELEK VSKNIGKIQV IEKTDEICPL CGSKLVIREN KNGKYYACSR 610 620 630 640 650 660 YPECKYTKPI YEEAQGRCPL CGGVVVKKKA KKTKKTFWGC INYPECSFAS PYEPLNEKCP 670 680 690 QCGSTMLKGN GFKKCSNKDC NYIVWTGRRK RWKKSK

References

[1]

"Complete sequence of Dictyoglomus turgidum DSM 6724." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Mead D. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z-1310 / DSM 6724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001251 Genomic DNA. Translation: ACK42800.1.
RefSeq	YP_002353414.1. NC_011661.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8E2F6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7081961.
GenomeReviews	Gene locus Dtur_1526 in contig CP001251_GR.
KEGG	dtu:Dtur_1526.
PATRIC	21811856. VBIDicTur93964_1575.
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	B8E2F6.
ProtClustDB	CLSK2410035.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8E2F6_DICTD

Accession

Primary (citable) accession number: B8E2F6

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)