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B8E181

- PNPH_DICTD

UniProt

B8E181 - PNPH_DICTD

Protein

Probable 6-oxopurine nucleoside phosphorylase

Gene

Dtur_0928

Organism
Dictyoglomus turgidum (strain Z-1310 / DSM 6724)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.UniRule annotation

    Catalytic activityi

    Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91PhosphateUniRule annotation
    Sitei164 – 1641Important for substrate specificityUniRule annotation
    Binding sitei181 – 1811Substrate; via amide nitrogenUniRule annotation
    Binding sitei182 – 1821PhosphateUniRule annotation
    Sitei217 – 2171Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
    3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

    GO - Biological processi

    1. purine ribonucleoside salvage Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciDTUR515635:GH4F-939-MONOMER.
    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
    Alternative name(s):
    Purine nucleoside phosphorylaseUniRule annotation
    Short name:
    PNPUniRule annotation
    Gene namesi
    Ordered Locus Names:Dtur_0928
    OrganismiDictyoglomus turgidum (strain Z-1310 / DSM 6724)
    Taxonomic identifieri515635 [NCBI]
    Taxonomic lineageiBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus
    ProteomesiUP000007719: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 263263Probable 6-oxopurine nucleoside phosphorylasePRO_0000415081Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi515635.Dtur_0928.

    Structurei

    3D structure databases

    ProteinModelPortaliB8E181.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 502Phosphate bindingUniRule annotation
    Regioni82 – 832Phosphate bindingUniRule annotation
    Regioni205 – 2073Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0005.
    HOGENOMiHOG000228987.
    KOiK00772.
    OMAiHVVGMTA.
    OrthoDBiEOG6KHFXC.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8E181-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIAIIGGTG VYDPKFLENP EEIKVSTPYG EVKLLKGIYQ GEEVGFLARH    50
    GAGHTVPPHR INYKANMWAL KSLGVERILS TTAVGSLKLN LVPGDLVILD 100
    QFIDFTKNRD HTFYNGDDGK VIHIDFTNPY CPELRNILYE TSKEIGIKAH 150
    PFGTYVCTEG PRFETPAEIK MYSFFGDVVG MTNVPEVILA RELEICYASV 200
    SLVTNYAAGI SPNPLTHSEV LEVMTQNIEK VRKLFAAVIP KIPKERNCIC 250
    KNALKEYREK GLL 263
    Length:263
    Mass (Da):29,405
    Last modified:March 3, 2009 - v1
    Checksum:i64B2331F6F124FE0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001251 Genomic DNA. Translation: ACK42209.1.
    RefSeqiYP_002352823.1. NC_011661.1.

    Genome annotation databases

    EnsemblBacteriaiACK42209; ACK42209; Dtur_0928.
    GeneIDi7082885.
    KEGGidtu:Dtur_0928.
    PATRICi21810560. VBIDicTur93964_0965.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001251 Genomic DNA. Translation: ACK42209.1 .
    RefSeqi YP_002352823.1. NC_011661.1.

    3D structure databases

    ProteinModelPortali B8E181.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 515635.Dtur_0928.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK42209 ; ACK42209 ; Dtur_0928 .
    GeneIDi 7082885.
    KEGGi dtu:Dtur_0928.
    PATRICi 21810560. VBIDicTur93964_0965.

    Phylogenomic databases

    eggNOGi COG0005.
    HOGENOMi HOG000228987.
    KOi K00772.
    OMAi HVVGMTA.
    OrthoDBi EOG6KHFXC.

    Enzyme and pathway databases

    UniPathwayi UPA00606 .
    BioCyci DTUR515635:GH4F-939-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01694. MTAP. 1 hit.
    PROSITEi PS01240. PNP_MTAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Dictyoglomus turgidum DSM 6724."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Mead D.
      Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Z-1310 / DSM 6724.

    Entry informationi

    Entry nameiPNPH_DICTD
    AccessioniPrimary (citable) accession number: B8E181
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3