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B8E181

- PNPH_DICTD

UniProt

B8E181 - PNPH_DICTD

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Protein
Probable 6-oxopurine nucleoside phosphorylase
Gene
Dtur_0928
Organism
Dictyoglomus turgidum (strain Z-1310 / DSM 6724)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage By similarity.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91Phosphate By similarity
Sitei164 – 1641Important for substrate specificity By similarity
Binding sitei181 – 1811Substrate; via amide nitrogen By similarity
Binding sitei182 – 1821Phosphate By similarity
Sitei217 – 2171Important for substrate specificity By similarity

GO - Molecular functioni

  1. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro
  2. phosphorylase activity Source: InterPro
  3. purine-nucleoside phosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. purine ribonucleoside salvage Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciDTUR515635:GH4F-939-MONOMER.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-oxopurine nucleoside phosphorylase (EC:2.4.2.1)
Alternative name(s):
Purine nucleoside phosphorylase
Short name:
PNP
Gene namesi
Ordered Locus Names:Dtur_0928
OrganismiDictyoglomus turgidum (strain Z-1310 / DSM 6724)
Taxonomic identifieri515635 [NCBI]
Taxonomic lineageiBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus
ProteomesiUP000007719: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation
PRO_0000415081Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi515635.Dtur_0928.

Structurei

3D structure databases

ProteinModelPortaliB8E181.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 502Phosphate binding By similarity
Regioni82 – 832Phosphate binding By similarity
Regioni205 – 2073Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
KOiK00772.
OMAiHVVGMTA.
OrthoDBiEOG6KHFXC.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8E181-1 [UniParc]FASTAAdd to Basket

« Hide

MRIAIIGGTG VYDPKFLENP EEIKVSTPYG EVKLLKGIYQ GEEVGFLARH    50
GAGHTVPPHR INYKANMWAL KSLGVERILS TTAVGSLKLN LVPGDLVILD 100
QFIDFTKNRD HTFYNGDDGK VIHIDFTNPY CPELRNILYE TSKEIGIKAH 150
PFGTYVCTEG PRFETPAEIK MYSFFGDVVG MTNVPEVILA RELEICYASV 200
SLVTNYAAGI SPNPLTHSEV LEVMTQNIEK VRKLFAAVIP KIPKERNCIC 250
KNALKEYREK GLL 263
Length:263
Mass (Da):29,405
Last modified:March 3, 2009 - v1
Checksum:i64B2331F6F124FE0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001251 Genomic DNA. Translation: ACK42209.1.
RefSeqiYP_002352823.1. NC_011661.1.

Genome annotation databases

EnsemblBacteriaiACK42209; ACK42209; Dtur_0928.
GeneIDi7082885.
KEGGidtu:Dtur_0928.
PATRICi21810560. VBIDicTur93964_0965.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001251 Genomic DNA. Translation: ACK42209.1 .
RefSeqi YP_002352823.1. NC_011661.1.

3D structure databases

ProteinModelPortali B8E181.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 515635.Dtur_0928.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK42209 ; ACK42209 ; Dtur_0928 .
GeneIDi 7082885.
KEGGi dtu:Dtur_0928.
PATRICi 21810560. VBIDicTur93964_0965.

Phylogenomic databases

eggNOGi COG0005.
HOGENOMi HOG000228987.
KOi K00772.
OMAi HVVGMTA.
OrthoDBi EOG6KHFXC.

Enzyme and pathway databases

UniPathwayi UPA00606 .
BioCyci DTUR515635:GH4F-939-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
TIGRFAMsi TIGR01694. MTAP. 1 hit.
PROSITEi PS01240. PNP_MTAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Dictyoglomus turgidum DSM 6724."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Mead D.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Z-1310 / DSM 6724.

Entry informationi

Entry nameiPNPH_DICTD
AccessioniPrimary (citable) accession number: B8E181
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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