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Protein

Probable 6-oxopurine nucleoside phosphorylase

Gene

Dtur_0928

Organism
Dictyoglomus turgidum (strain Z-1310 / DSM 6724)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: purine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9PhosphateUniRule annotation1
Sitei164Important for substrate specificityUniRule annotation1
Binding sitei181Substrate; via amide nitrogenUniRule annotation1
Binding sitei182PhosphateUniRule annotation1
Sitei217Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciDTUR515635:GH4F-878-MONOMER.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
Alternative name(s):
Purine nucleoside phosphorylaseUniRule annotation
Short name:
PNPUniRule annotation
Gene namesi
Ordered Locus Names:Dtur_0928
OrganismiDictyoglomus turgidum (strain Z-1310 / DSM 6724)
Taxonomic identifieri515635 [NCBI]
Taxonomic lineageiBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus
Proteomesi
  • UP000007719 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004150811 – 263Probable 6-oxopurine nucleoside phosphorylaseAdd BLAST263

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi515635.Dtur_0928.

Structurei

3D structure databases

ProteinModelPortaliB8E181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 50Phosphate bindingUniRule annotation2
Regioni82 – 83Phosphate bindingUniRule annotation2
Regioni205 – 207Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CB1. Bacteria.
COG0005. LUCA.
HOGENOMiHOG000228987.
InParanoidiB8E181.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiPOG091H0228.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP. 1 hit.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8E181-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIAIIGGTG VYDPKFLENP EEIKVSTPYG EVKLLKGIYQ GEEVGFLARH
60 70 80 90 100
GAGHTVPPHR INYKANMWAL KSLGVERILS TTAVGSLKLN LVPGDLVILD
110 120 130 140 150
QFIDFTKNRD HTFYNGDDGK VIHIDFTNPY CPELRNILYE TSKEIGIKAH
160 170 180 190 200
PFGTYVCTEG PRFETPAEIK MYSFFGDVVG MTNVPEVILA RELEICYASV
210 220 230 240 250
SLVTNYAAGI SPNPLTHSEV LEVMTQNIEK VRKLFAAVIP KIPKERNCIC
260
KNALKEYREK GLL
Length:263
Mass (Da):29,405
Last modified:March 3, 2009 - v1
Checksum:i64B2331F6F124FE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001251 Genomic DNA. Translation: ACK42209.1.
RefSeqiWP_012583293.1. NC_011661.1.
YP_002352823.1. NC_011661.1.

Genome annotation databases

EnsemblBacteriaiACK42209; ACK42209; Dtur_0928.
GeneIDi7082885.
KEGGidtu:Dtur_0928.
PATRICi21810560. VBIDicTur93964_0965.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001251 Genomic DNA. Translation: ACK42209.1.
RefSeqiWP_012583293.1. NC_011661.1.
YP_002352823.1. NC_011661.1.

3D structure databases

ProteinModelPortaliB8E181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi515635.Dtur_0928.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK42209; ACK42209; Dtur_0928.
GeneIDi7082885.
KEGGidtu:Dtur_0928.
PATRICi21810560. VBIDicTur93964_0965.

Phylogenomic databases

eggNOGiENOG4105CB1. Bacteria.
COG0005. LUCA.
HOGENOMiHOG000228987.
InParanoidiB8E181.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiPOG091H0228.

Enzyme and pathway databases

UniPathwayiUPA00606.
BioCyciDTUR515635:GH4F-878-MONOMER.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP. 1 hit.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNPH_DICTD
AccessioniPrimary (citable) accession number: B8E181
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.