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B8E181 (PNPH_DICTD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 6-oxopurine nucleoside phosphorylase

EC=2.4.2.1
Alternative name(s):
Purine nucleoside phosphorylase
Short name=PNP
Gene names
Ordered Locus Names:Dtur_0928
OrganismDictyoglomus turgidum (strain Z-1310 / DSM 6724) [Reference proteome] [HAMAP]
Taxonomic identifier515635 [NCBI]
Taxonomic lineageBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage By similarity. HAMAP-Rule MF_01963

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01963

Pathway

Purine metabolism; purine nucleoside salvage. HAMAP-Rule MF_01963

Subunit structure

Homohexamer. Dimer of a homotrimer By similarity. HAMAP-Rule MF_01963

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263Probable 6-oxopurine nucleoside phosphorylase HAMAP-Rule MF_01963
PRO_0000415081

Regions

Region49 – 502Phosphate binding By similarity
Region82 – 832Phosphate binding By similarity
Region205 – 2073Substrate binding By similarity

Sites

Binding site91Phosphate By similarity
Binding site1811Substrate; via amide nitrogen By similarity
Binding site1821Phosphate By similarity
Site1641Important for substrate specificity By similarity
Site2171Important for substrate specificity By similarity

Sequences

Sequence LengthMass (Da)Tools
B8E181 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 64B2331F6F124FE0

FASTA26329,405
        10         20         30         40         50         60 
MRIAIIGGTG VYDPKFLENP EEIKVSTPYG EVKLLKGIYQ GEEVGFLARH GAGHTVPPHR 

        70         80         90        100        110        120 
INYKANMWAL KSLGVERILS TTAVGSLKLN LVPGDLVILD QFIDFTKNRD HTFYNGDDGK 

       130        140        150        160        170        180 
VIHIDFTNPY CPELRNILYE TSKEIGIKAH PFGTYVCTEG PRFETPAEIK MYSFFGDVVG 

       190        200        210        220        230        240 
MTNVPEVILA RELEICYASV SLVTNYAAGI SPNPLTHSEV LEVMTQNIEK VRKLFAAVIP 

       250        260 
KIPKERNCIC KNALKEYREK GLL 

« Hide

References

[1]"Complete sequence of Dictyoglomus turgidum DSM 6724."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Mead D.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Z-1310 / DSM 6724.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001251 Genomic DNA. Translation: ACK42209.1.
RefSeqYP_002352823.1. NC_011661.1.

3D structure databases

ProteinModelPortalB8E181.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING515635.Dtur_0928.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK42209; ACK42209; Dtur_0928.
GeneID7082885.
KEGGdtu:Dtur_0928.
PATRIC21810560. VBIDicTur93964_0965.

Phylogenomic databases

eggNOGCOG0005.
HOGENOMHOG000228987.
KOK00772.
OMAHVVGMTA.
OrthoDBEOG6KHFXC.

Enzyme and pathway databases

BioCycDTUR515635:GH4F-939-MONOMER.
UniPathwayUPA00606.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR01694. MTAP. 1 hit.
PROSITEPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNPH_DICTD
AccessionPrimary (citable) accession number: B8E181
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways