B8DWR0 (SYR_BIFA0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	BLA_0618

Organism

Bifidobacterium animalis subsp. lactis (strain AD011) [Complete proteome] [HAMAP]

Taxonomic identifier

442563 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium › Bifidobacterium animalis › Bifidobacterium animalis subsp. lactis

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_1000198874
Regions
	Motif	135 – 145	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B8DWR0 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 7D962679DCF11F41	FASTA	598	65,586
	10 20 30 40 50 60 MSPEALQELI FTIANNLVSE GKAGTLTAEE LPDSAKFAVM RPKDRAHGDW ASNAAMQLAK 70 80 90 100 110 120 KAGMKPRDLA QLFADALNGT DGIAAVEVAG PGFINITLDS ASAAAVVDQV LDEGNRFGKN 130 140 150 160 170 180 NHLSGKTLNL EFVSANPTGP IHIGGTRWAA VGDSMARILQ ANGATVVREY YFNDHGEQIN 190 200 210 220 230 240 RFAKSLVAAA HDEPTPVDGY KGAYIDEIAR RVIVEANAEG IDILNLPRVD GGTDEKGEPL 250 260 270 280 290 300 GEGDSEQREE FRKRAVPMMF DEIRQSMKEF RVHFDVWFHE NSLYEDGEVE KAIADLRNAG 310 320 330 340 350 360 DIYEKDGATW FESTEHGDDK DRVIIKSDGT YAYFAADIAY YRNKRHRKTD PADVAIYMLG 370 380 390 400 410 420 ADHHGYIGRM MAMCAAFGDK PGENMQILIG QMVNVMKDGK PVRMSKRAGN IVTIDDLIDA 430 440 450 460 470 480 IGVDASRYSL ARTDYNSPVD IDLNLLASHS NDNPVYYVQY AHARSCNVDR NAEAAQINAA 490 500 510 520 530 540 DADLSLLDTE ADGEVIAALA QWPALLTLAG DLRAPHRIAH YLEDLAAAYH KWYNVERVVP 550 560 570 580 590 MPLTEAEERA DEQTRERTRI AKNPEPARAA ARLKLNDAVQ TVIAEGLDLL GVTAPDKM

References

[1]

"Genome sequence of the probiotic bacterium Bifidobacterium animalis subsp. lactis AD011." Kim J.F., Jeong H., Yu D.S., Choi S.-H., Hur C.-G., Park M.-S., Yoon S.H., Kim D.-W., Ji G.E., Park H.-S., Oh T.K. J. Bacteriol. 191:678-679(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AD011.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001213 Genomic DNA. Translation: ACL28911.1.
RefSeq	YP_002469487.1. NC_011835.1.
3D structure databases
ProteinModelPortal	B8DWR0.
ModBase	Search...
Protein-protein interaction databases
STRING	442563.BLA_0618.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL28911; ACL28911; BLA_0618.
GeneID	7264025.
KEGG	bla:BLA_0618.
PATRIC	21104121. VBIBifAni98964_0649.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247214.
KO	K01887.
OMA	HTSANPN.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	BANI442563:GHG0-613-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_BIFA0

Accession

Primary (citable) accession number: B8DWR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program