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B8DWI2 (DAPA_BIFA0) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:BLA_0534
OrganismBifidobacterium animalis subsp. lactis (strain AD011) [Complete proteome] [HAMAP]
Taxonomic identifier442563 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303Dihydrodipicolinate synthase HAMAP MF_00418
PRO_1000134858

Regions

Region60 – 612Pyruvate binding By similarity

Sites

Active site1711Schiff-base intermediate with substrate By similarity
Binding site1161Pyruvate By similarity
Site1431Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
B8DWI2 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 1BEC4403872EAA5E

FASTA30332,204
        10         20         30         40         50         60 
MSDGIMHLLD PAPFGRVLPA MITPMKPNGD VDFDMAQTVA KQLVADGADG LVVNGTTGES 

        70         80         90        100        110        120 
PTTHMDEKVE LVKAVKEVVD VPVISGAGSN DTAHTVRMVE QTQEAGADAV LVVCPYYSRP 

       130        140        150        160        170        180 
SQQGIFCHYQ AVNESADKPI IVYDVPGRTG VRIALDTYVH LAELDHVKAV KDATGDIAGA 

       190        200        210        220        230        240 
VRKRMETGLT WYSGDDALYL PFLSIGAVGI ISVVAHAAAK PMRELAEAFD RGDIAKAQGL 

       250        260        270        280        290        300 
ANRIAPVIEA MNGTGFQAVM AKAALKVRGI MECTTMRLPN IGPNDEQIEV VRDGLRASGL 


IPE

« Hide

References

[1]"Genome sequence of the probiotic bacterium Bifidobacterium animalis subsp. lactis AD011."
Kim J.F., Jeong H., Yu D.S., Choi S.-H., Hur C.-G., Park M.-S., Yoon S.H., Kim D.-W., Ji G.E., Park H.-S., Oh T.K.
J. Bacteriol. 191:678-679(2009) [PubMed: 19011029] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AD011.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001213 Genomic DNA. Translation: ACL28833.1.
RefSeqYP_002469409.1. NC_011835.1.

3D structure databases

ProteinModelPortalB8DWI2.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8DWI2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7263944.
GenomeReviewsGene locus BLA_0534 in contig CP001213_GR.
KEGGbla:BLA_0534.
PATRIC21103949. VBIBifAni98964_0563.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG358848.
OMAHGCISVV.
ProtClustDBPRK03170.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_BIFA0
AccessionPrimary (citable) accession number: B8DWI2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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