ID   B8DUR9_BIFA0            Unreviewed;       500 AA.
AC   B8DUR9;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   27-MAR-2024, entry version 72.
DE   SubName: Full=Alpha-amylase {ECO:0000313|EMBL:ACL29748.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:ACL29748.1};
GN   Name=amy {ECO:0000313|EMBL:ACL29748.1};
GN   OrderedLocusNames=BLA_1466 {ECO:0000313|EMBL:ACL29748.1};
OS   Bifidobacterium animalis subsp. lactis (strain AD011).
OC   Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC   Bifidobacteriaceae; Bifidobacterium.
OX   NCBI_TaxID=442563 {ECO:0000313|EMBL:ACL29748.1, ECO:0000313|Proteomes:UP000002456};
RN   [1] {ECO:0000313|EMBL:ACL29748.1, ECO:0000313|Proteomes:UP000002456}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AD011 {ECO:0000313|EMBL:ACL29748.1,
RC   ECO:0000313|Proteomes:UP000002456};
RX   PubMed=19011029; DOI=10.1128/JB.01515-08;
RA   Kim J.F., Jeong H., Yu D.S., Choi S.-H., Hur C.-G., Park M.-S., Yoon S.H.,
RA   Kim D.-W., Ji G.E., Park H.-S., Oh T.K.;
RT   "Genome sequence of the probiotic bacterium Bifidobacterium animalis subsp.
RT   lactis AD011.";
RL   J. Bacteriol. 191:678-679(2009).
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|RuleBase:RU003615}.
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DR   EMBL; CP001213; ACL29748.1; -; Genomic_DNA.
DR   RefSeq; WP_004217797.1; NC_011835.1.
DR   AlphaFoldDB; B8DUR9; -.
DR   STRING; 442563.BLA_1466; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   GeneID; 66533296; -.
DR   KEGG; bla:BLA_1466; -.
DR   HOGENOM; CLU_024572_2_0_11; -.
DR   Proteomes; UP000002456; Chromosome.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:InterPro.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR   Gene3D; 2.40.30.140; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR006046; Alpha_amylase.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   PRINTS; PR00110; ALPHAAMYLASE.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000313|EMBL:ACL29748.1};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ACL29748.1};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002456}.
FT   DOMAIN          9..394
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   ACT_SITE        236
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   ACT_SITE        266
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   BINDING         109
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         199
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         205
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         207
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ   SEQUENCE   500 AA;  56022 MW;  7173B532435E5BD5 CRC64;
     MAIDKPNHET MLQCFEWYLP ESHNLWRWLS SQAPSVAHAG FTTAWLPPAY KGQAGDSDVG
     YGVYDMYDLG EFDAKGSVPT KYGSRMEYLQ AIRAMQGNGV RVFADIVFNH RMGADGTEPV
     RTHEVNVDDR TRSDSTVVER TLNTVYDFPE RGGVYSTFKW NWSDFTGTDY TTDDGTTGIM
     RFDGKQWSDN VSHERGNFDY IMGDDVDVNE PEVARELTDW GIWYTTTTGV DGFRLDAVKS
     IDAGFFAPWL RTMQRYGNHP GIAVGEYWSG DASELTSYLH DCNHCMMLFD VALHFRFEQA
     SKNPEGFDLR GLAADTLYER EPTYACTFVD NHDTQPGQAL ESWVQPWFKP LAYACILLRD
     NVLPCVFFGD YYGVPHDLIP PMRFLPHMVW IRAHLLGDQV EAQPGDTAHS LCWVVEGNHP
     VCVVLNTGSS EVHRQVRNAA LASHTLIDVC HPDAPATTDT QGQGMMRCPP RSCAIYIDAD
     DYGVMLEALS GTPAAGTVCA
//