B8DUK6 (METE_BIFA0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	BLA_1400

Organism

Bifidobacterium animalis subsp. lactis (strain AD011) [Complete proteome] [HAMAP]

Taxonomic identifier

442563 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000123778
Sites
	Metal binding	653	1	Zinc By similarity
	Metal binding	655	1	Zinc By similarity
	Metal binding	738	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8DUK6 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 97D9FF6BD4D45AB4	FASTA	767	86,685
	10 20 30 40 50 60 MTIPTSISGF PRIGRDRELK KAIEAYWKGN ATLDDVRETG RELRVRHWRL EKDAGIDLIP 70 80 90 100 110 120 SNDFSLYDQM LDTAVLLNVI PARYRRLGFD NPEETLFAMA RGYQGERGDV TALPMKKWFT 130 140 150 160 170 180 TNYHYLVPEF DSSTEVRLNG TKPFDEYLEA KSLGIETKPV LIGPYTFLKL SRDAEAQELT 190 200 210 220 230 240 IDEGLVDAVA DVYAEVLRRF AKLGAQWVQL DEPYLVLDKH EGDVSLFKSL YSRLLPARDS 250 260 270 280 290 300 GVRVLLNTYF GNIADIYETV KLFEFDGVGL DLIEGRDENL KAVEHHGVAA RTTLFAGVVN 310 320 330 340 350 360 GRNIWRNNYA ESIALVEALQ QVTANVAVAS ACSLLHVPFS TKGEEQLGDK VLRHFAFAVE 370 380 390 400 410 420 KLEEVREIAD LMQLDDDAKR QSTALAANQA LFDGSRVVAD EVVRARIAAL ADADYVRRPE 430 440 450 460 470 480 RGERQRLQRE ALGLPLLPTT TIGSFPQTKQ VRAERARLRK GEISQGQYDE FIRRQIDDVV 490 500 510 520 530 540 AKQEQIGLDV LVHGEFERND MVEYFGQNLN GFLFTKNAWV QSYGTRCVKP PIIWGDVSRA 550 560 570 580 590 600 QPITVAWSSY AQSRTAKPMK GMLTGPVTIL NWSWPREDIT HEEQTKQLAL AIRDEVLDLE 610 620 630 640 650 660 KAGIRIIQID EAALREKLPL RESDWHREYL DWAIPAFRLV HSAVQPSTQI HTHMCYSEFN 670 680 690 700 710 720 DIISDIDAMD ADVISFEASR GDLVVLDAIH DAHFETEAGP GVYDIHSPRI PSVEETEERI 730 740 750 760 GESLAKMDVN KVWINPDCGL KTRGNDETWP SLTHMVQAAK AMRANLD

References

[1]

"Genome sequence of the probiotic bacterium Bifidobacterium animalis subsp. lactis AD011." Kim J.F., Jeong H., Yu D.S., Choi S.-H., Hur C.-G., Park M.-S., Yoon S.H., Kim D.-W., Ji G.E., Park H.-S., Oh T.K. J. Bacteriol. 191:678-679(2009) [PubMed: 19011029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AD011.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001213 Genomic DNA. Translation: ACL29685.1.
RefSeq	YP_002470261.1. NC_011835.1.
3D structure databases
ProteinModelPortal	B8DUK6.
ModBase	Search...
Protein-protein interaction databases
STRING	B8DUK6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7264804.
GenomeReviews	Gene locus BLA_1400 in contig CP001213_GR.
KEGG	bla:BLA_1400.
PATRIC	21105810. VBIBifAni98964_1464.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BIFA0

Accession

Primary (citable) accession number: B8DUK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program