Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B8DPP9

- BIOB_DESVM

UniProt

B8DPP9 - BIOB_DESVM

Protein

Biotin synthase

Gene

bioB

Organism
Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi88 – 881Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi164 – 1641Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi233 – 2331Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciDVUL883:GCJ5-1030-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:DvMF_1001
    OrganismiDesulfovibrio vulgaris (strain Miyazaki F / DSM 19637)
    Taxonomic identifieri883 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
    ProteomesiUP000001361: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 364364Biotin synthasePRO_0000381352Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi883.DvMF_1001.

    Structurei

    3D structure databases

    ProteinModelPortaliB8DPP9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B8DPP9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPLLERLCA RLSDTIPPGT ETPYASPSHA RTEEAPWSGI TGEEALAVAR    50
    LPASDILDIL AVAQAVRSAR KGPLATTCGI VNAKSGRCGE DCAFCAQSSH 100
    HDTGAPVHAL LGPDALLRHA EELARAGVRR FGIVTSGNAL SEREFDAVCH 150
    AARLLRDRVD IGLCASLGQL ATGSAESGNR GERARRLKDA GISSYHHNLE 200
    TARSFFPQVC TTHPYDDDIA TVREAARAGL RTCCGGILGL GETWEHRVEL 250
    ALTLRELDVD SIPLNFLHPV PGTRLGHRSP LPPMEALRAI AVFRLLHPQR 300
    DILVCGGRET TLGQWQSWVF AAGANGLMVG NYLTTAGRAL AEDMEMLAAL 350
    GVGEIPRNGE EARA 364
    Length:364
    Mass (Da):38,891
    Last modified:March 3, 2009 - v1
    Checksum:i765502FEC70944AD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001197 Genomic DNA. Translation: ACL07956.1.
    RefSeqiWP_012612145.1. NC_011769.1.
    YP_002435424.1. NC_011769.1.

    Genome annotation databases

    EnsemblBacteriaiACL07956; ACL07956; DvMF_1001.
    GeneIDi7172896.
    KEGGidvm:DvMF_1001.
    PATRICi21772033. VBIDesVul86729_1059.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001197 Genomic DNA. Translation: ACL07956.1 .
    RefSeqi WP_012612145.1. NC_011769.1.
    YP_002435424.1. NC_011769.1.

    3D structure databases

    ProteinModelPortali B8DPP9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 883.DvMF_1001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL07956 ; ACL07956 ; DvMF_1001 .
    GeneIDi 7172896.
    KEGGi dvm:DvMF_1001.
    PATRICi 21772033. VBIDesVul86729_1059.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci DVUL883:GCJ5-1030-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Desulfovibrio vulgaris str. 'Miyazaki F'."
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Hazen T.C., Richardson P.
      Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Miyazaki F / DSM 19637.

    Entry informationi

    Entry nameiBIOB_DESVM
    AccessioniPrimary (citable) accession number: B8DPP9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3