B8DMM2 (METE_DESVM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	DvMF_2035

Organism

Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637) [Complete proteome] [HAMAP]

Taxonomic identifier

883 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	780 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 780	780	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000191196
Sites
	Metal binding	667	1	Zinc By similarity
	Metal binding	669	1	Zinc By similarity
	Metal binding	752	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8DMM2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 78986F9D1A868056	FASTA	780	84,734
	10 20 30 40 50 60 MRTHTLGFPR IGGNRELKKA VEDYWKGAAT RQQLEDAARA IRLRNWTLQR EAGIDVVPVG 70 80 90 100 110 120 DFALYDHILD AALLLGVIPP RFRNDDAPRD IDLMFRMARG QGGDRPVAPL EMTKWFDTNY 130 140 150 160 170 180 HYLVPELDAA TTFAPDAAPL LALIDEALAA GFTPKAVLPG PMTFLWLSKR VDGGTRWSLL 190 200 210 220 230 240 PALLDAYGAL LRDVAARCPL IQLDEPILSL DLADDIRSRF VPAYARLRVA VPDATLLLAS 250 260 270 280 290 300 YFAPVGDNLP VALSLPVDVV HLDLVRGPED LTPALDILTR AAARQPADSG LPQSGSQSGI 310 320 330 340 350 360 ALSLGVINGR NVWRVDADKA AAPVRAAVAA LGPDRVWVAP SCSLLHCPVD LDAETGLDPE 370 380 390 400 410 420 VAQWLAFARQ KCAEVRLVAD MCDLNGRADA PETAAALARN RAALAARAAS PVIHDPAVAV 430 440 450 460 470 480 RAGTVTPEMG WRATPYTARI AAQRAALRLP VLPATTIGSF PQTVDIRAQR RKLRTGQITE 490 500 510 520 530 540 AQYDAAMREA IATAIREQEA LGLDVLVHGE PERNDMVEYF GEQLRGFCIT ANGWVQSYGT 550 560 570 580 590 600 RCVKPPLLYG DVARPGPMTV RWITHAQSLT QKPVKGMLTG PVTIACWSFV RDDVPRPVVF 610 620 630 640 650 660 RQLALAIRDE VADLEAAGIG VIQIDEPALR EGLPLRRAAH AAYLDAAVGA FRLASSGVRD 670 680 690 700 710 720 ATQIHTHMCY CDFHDIIDHV AALDADVISL EASRSRMELL DVFATHAYPN EVGPGVYDIH 730 740 750 760 770 780 SPRVPSIDEM ETLLRAASRV LPLDRLWANP DCGLKTRDWP ETRASLAHLV AAATRVREGG

References

[1]

"Complete sequence of Desulfovibrio vulgaris str. 'Miyazaki F'." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Hazen T.C., Richardson P. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Miyazaki F / DSM 19637.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001197 Genomic DNA. Translation: ACL08978.1.
RefSeq	YP_002436446.1. NC_011769.1.
3D structure databases
ProteinModelPortal	B8DMM2.
ModBase	Search...
Protein-protein interaction databases
STRING	B8DMM2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7173954.
GenomeReviews	Gene locus DvMF_2035 in contig CP001197_GR.
KEGG	dvm:DvMF_2035.
PATRIC	21774321. VBIDesVul86729_2180.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_DESVM

Accession

Primary (citable) accession number: B8DMM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program