B8DM91 (LEU1_DESVM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-isopropylmalate synthase
EC=2.3.3.13

Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase

Gene names

Name:	leuA
Ordered Locus Names:	DvMF_1791

Organism

Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637) [Complete proteome] [HAMAP]

Taxonomic identifier

883 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › Desulfovibrio vulgaris

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_01025
Catalytic activity	Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_01025
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_01025
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-isopropylmalate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	2-isopropylmalate synthase HAMAP-Rule MF_01025		PRO_1000149178

Sequences

	Sequence		Length	Mass (Da)
	B8DM91 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 0D5E34397B4CB55F	FASTA	514	55,900
	10 20 30 40 50 60 MTIESGRIRI FDTTLRDGEQ SPGATMNLQE KIRLARQLET LGVDIMEAGF PASSQGDFEA 70 80 90 100 110 120 VQAIARAVKG VEVAGLCRAM PADIDRAWEA VKVAENPRIH TFLATSPVHM QYKLRKEPDQ 130 140 150 160 170 180 VVEMAVAAVR HAAKYTSNVE FSAEDASRSN PDFLVRVFEA VINAGATTIN VPDTVGYAQP 190 200 210 220 230 240 EEFGRLIRYV IENTPNSHKA VFSVHCHNDL GMGVANTLAA LKAGARQAEV TISGIGERAG 250 260 270 280 290 300 NASLEEIVMA LHTRRDFYQL DCGVVTEQLF PTCRLLSMII GQPIPPNKAI VGANAFAHES 310 320 330 340 350 360 GIHQDGMLKN RETYEIMTPE SIGKTKTDLV IGKHSGRNAV KNKLDELGYR LEEAQLVTVF 370 380 390 400 410 420 EAVKKLADKK KQIYDEDIEA LVLEEVYRLP DLYRLVNLSV QCSDTGMPPT AAVVMDVMGE 430 440 450 460 470 480 VKRAAGFGVG PIDAVFNVIG EIVGRAPVLE RYSVTAITGG TDAQGEVTVR LRQNGSSAVG 490 500 510 RGSDPDIILA SARAYVNALN RLAKKEEEQE KEGI

References

[1]

"Complete sequence of Desulfovibrio vulgaris str. 'Miyazaki F'." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Hazen T.C., Richardson P. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Miyazaki F / DSM 19637.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001197 Genomic DNA. Translation: ACL08735.1.
RefSeq	YP_002436203.1. NC_011769.1.
3D structure databases
ProteinModelPortal	B8DM91.
ModBase	Search...
Protein-protein interaction databases
STRING	883.DvMF_1791.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL08735; ACL08735; DvMF_1791.
GeneID	7173707.
KEGG	dvm:DvMF_1791.
PATRIC	21773759. VBIDesVul86729_1902.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0119.
HOGENOM	HOG000046859.
KO	K01649.
OMA	ITDRGEN.
ProtClustDB	PRK00915.
Enzyme and pathway databases
UniPathway	UPA00048; UER00070.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01025. LeuA_type1.
InterPro	IPR013709. 2-isopropylmalate_synth_dimer. IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR005671. LeuA_bact_synth. IPR000891. PYR_CT. [Graphical view]
Pfam	PF00682. HMGL-like. 1 hit. PF08502. LeuA_dimer. 1 hit. [Graphical view]
SMART	SM00917. LeuA_dimer. 1 hit. [Graphical view]
SUPFAM	SSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMs	TIGR00973. leuA_bact. 1 hit.
PROSITE	PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit. PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU1_DESVM

Accession

Primary (citable) accession number: B8DM91

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program