B8DKP2 (PHNX_DESVM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphonoacetaldehyde hydrolase
Short name=Phosphonatase
EC=3.11.1.1

Alternative name(s):
Phosphonoacetaldehyde phosphonohydrolase

Gene names

Name:	phnX
Ordered Locus Names:	DvMF_0514

Organism

Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637) [Complete proteome] [HAMAP]

Taxonomic identifier

883 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › Desulfovibrio vulgaris

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in phosphonate degradation By similarity.
Catalytic activity	Phosphonoacetaldehyde + H2O = acetaldehyde + phosphate. HAMAP-Rule MF_01375
Cofactor	Binds 1 Mg2+ ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PhnX family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Schiff base
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	organic phosphonate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: InterPro phosphonoacetaldehyde hydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Phosphonoacetaldehyde hydrolase HAMAP-Rule MF_01375		PRO_1000144832
Sites
	Active site	20	1	Nucleophile By similarity
	Active site	61	1	Schiff-base intermediate with substrate By similarity
	Metal binding	20	1	Magnesium By similarity
	Metal binding	22	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	194	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8DKP2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 102D065062AACB1E	FASTA	280	30,244
	10 20 30 40 50 60 MKPFLRTRVY DGPVRAVVLD WAGTAVDHGC LGPAAVFVQA FALHGVEVAV SEAREPMGSE 70 80 90 100 110 120 KREHVRRMLA MDSVAARWRA VHGHVPHEAD VDAVYRDVEP LMLQTIAAHA VPVPGLAEFV 130 140 150 160 170 180 DRVRGRGMGL GSCTGYTGPM MEVLVPEAAR RGYSPDVVVH ASEVPAGRPY PWMCYLNAMR 190 200 210 220 230 240 LGVHPMESMV KIGDTVADMH EARNAGMWTV GVVRTGNDVG LSEVDAARMP PDQLAARMTV 250 260 270 280 AAARLREAGA HYVVDSIADC FSVIKAIEAR LARGDTPYPA

References

[1]

"Complete sequence of Desulfovibrio vulgaris str. 'Miyazaki F'." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Hazen T.C., Richardson P. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Miyazaki F / DSM 19637.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001197 Genomic DNA. Translation: ACL07471.1.
RefSeq	YP_002434939.1. NC_011769.1.
3D structure databases
ProteinModelPortal	B8DKP2.
ModBase	Search...
Protein-protein interaction databases
STRING	883.DvMF_0514.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL07471; ACL07471; DvMF_0514.
GeneID	7172401.
KEGG	dvm:DvMF_0514.
PATRIC	21770981. VBIDesVul86729_0541.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0637.
HOGENOM	HOG000217971.
KO	K05306.
OMA	EFGATWE.
ProtClustDB	PRK13478.
Family and domain databases
Gene3D	1.10.150.240. 1 hit. 3.40.50.1000. 2 hits.
HAMAP	MF_01375. PhnX.
InterPro	IPR023214. HAD-like_dom. IPR006439. HAD-SF_hydro_IA_v1. IPR023198. PGP_dom2. IPR006323. Phosphonoacetald_hydro. [Graphical view]
Pfam	PF13419. HAD_2. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01549. HAD-SF-IA-v1. 1 hit. TIGR01422. phosphonatase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNX_DESVM

Accession

Primary (citable) accession number: B8DKP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program