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B8DJF0 (SYL_DESVM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:DvMF_3090
OrganismDesulfovibrio vulgaris (strain Miyazaki F / DSM 19637) [Complete proteome] [HAMAP]
Taxonomic identifier883 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 834834Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199197

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif586 – 5905"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5891ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B8DJF0 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: F3DE26FD5DD02836

FASTA83493,505
        10         20         30         40         50         60 
MKYDHQSIEI KWQRTWEESG AFHCDHHSDK PKYYVLEMFP YPSGNIHMGH VRNYSIGDVV 

        70         80         90        100        110        120 
ARFKRMQGFN VLHPMGWDAF GLPAENAAIK HGTHPAKWTF SNIDNMRTQL RRLGYSYDWR 

       130        140        150        160        170        180 
RELATCTPEY YRWEQQFFLR FFEKGLVYRK QAPQNWCPKC HTVLANEQVI EGLCWRCDSA 

       190        200        210        220        230        240 
VEQKNLTQWF LRITDYAEEL LADLDKLEAG WPERVVSMQR NWIGKSIGAE IVFPLEGPGG 

       250        260        270        280        290        300 
SGNDDSITVF TTRQDTVFGA TFMSLAPEHP LVEQLIDGKP EAPAVRAFVE RIRNMDRIVR 

       310        320        330        340        350        360 
QSDDLEKEGV FTGAYCVNPF TGRRMPIWVA NFVLAEYGTG AVMAVPAHDQ RDFEFARKYD 

       370        380        390        400        410        420 
LPMQVVIQPE GDALDTAAMQ AAWTEAGLLV NSGEFDGLPN EAAKQKIADS LETSGKGRRT 

       430        440        450        460        470        480 
VNYRLRDWNI SRQRYWGAPI PVVYCDACGV VAEKDENLPV LLPLEVRTHE DGRSPLPDTP 

       490        500        510        520        530        540 
EFAECACPKC GGKARRETDT MDTFVESSWY FARYTSASKD DGAFDPAALK YWMPVDQYIG 

       550        560        570        580        590        600 
GVEHAILHLL YSRFFVKALR DCGYMDLDEP FANLLTQGMV LKEGAKMSKS KGNVVDPTEM 

       610        620        630        640        650        660 
IARYGADTVR LFCLFAAPPE RDFDWSDSGI EGSYRFIGRI WRLAEELSGV LLPVKACSAT 

       670        680        690        700        710        720 
AADAATPQGK DLRNKEHATV RKAGADISDR FQFNTAIAAV MELVNALYLA KDELSGDEGG 

       730        740        750        760        770        780 
RKVLSSAVAT VLTLLSPITP HIAEELWASI GNAGRITDEP WPQWSEEALA RDEETIVVQI 

       790        800        810        820        830 
NGKLRGRVSV PAGADAKAIE AAALSEPNVA RHLEDVTVRK VVVIPGKLVN VVVG 

« Hide

References

[1]"Complete sequence of Desulfovibrio vulgaris str. 'Miyazaki F'."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Hazen T.C., Richardson P.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Miyazaki F / DSM 19637.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001197 Genomic DNA. Translation: ACL10027.1.
RefSeqYP_002437495.1. NC_011769.1.

3D structure databases

ProteinModelPortalB8DJF0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING883.DvMF_3090.

Proteomic databases

PRIDEB8DJF0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL10027; ACL10027; DvMF_3090.
GeneID7175036.
KEGGdvm:DvMF_3090.
PATRIC21776719. VBIDesVul86729_3352.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycDVUL883:GCJ5-3170-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_DESVM
AccessionPrimary (citable) accession number: B8DJF0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries