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B8DHL8 (TGT_LISMH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene names
Name:tgt
Ordered Locus Names:LMHCC_1039
OrganismListeria monocytogenes serotype 4a (strain HCC23) [Complete proteome] [HAMAP]
Taxonomic identifier552536 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria ›

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168

Catalytic activity

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

queuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168
PRO_1000198014

Sites

Active site941Nucleophile By similarity
Metal binding3061Zinc By similarity
Metal binding3081Zinc By similarity
Metal binding3111Zinc By similarity
Metal binding3371Zinc By similarity
Binding site951Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B8DHL8 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 570CCB198D2EC65B

FASTA37942,951
        10         20         30         40         50         60 
MSAIRYELIK TDKQTGARLG KIHTPHGTFD TPMFMPVGTL ATVKTMSPEE LKAMGAGIIL 

        70         80         90        100        110        120 
SNTYHLWLRP GEELIREAGG LHKFMNWDQP ILTDSGGFQV FSLSKMRDIK EEGVHFRNHL 

       130        140        150        160        170        180 
NGDKLFLSPE KAIQIQNALG SDIMMSFDEC PPYPASHEYM KKSVERTSRW AERGLKAHER 

       190        200        210        220        230        240 
PEDQGLFGIV QGGAYEDLRA QSAKDLVSLD FPGYSIGGLS VGEPKDVMNR VLEHTTPLLP 

       250        260        270        280        290        300 
ANKPRYLMGV GSPDSLIDGV IRGVDMFDCV LPTRIARNGT CMTSSGRLVI KNAKFTHDFR 

       310        320        330        340        350        360 
PIDENCDCYT CKNYSRAYIR HLIRCEETFG IRLTTYHNLH FLLNLMKQVR GAIMEDRLAD 

       370 
FREEFFEQYG FNRPDAKNF

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References

[1]"Genome sequence of lineage III Listeria monocytogenes strain HCC23."
Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T., Bridges S.M., Lawrence M.L.
J. Bacteriol. 193:3679-3680(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HCC23.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001175 Genomic DNA. Translation: ACK39387.1.
RefSeqYP_002350001.1. NC_011660.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING552536.LMHCC_1039.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK39387; ACK39387; LMHCC_1039.
GeneID7079972.
KEGGlmh:LMHCC_1039.
PATRIC20317195. VBILisMon86872_1028.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycLMON552536:GIW4-1075-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTGT_LISMH
AccessionPrimary (citable) accession number: B8DHL8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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