true2009-07-282024-03-2781HEM1_LISMHGenome sequence of lineage III Listeria monocytogenes strain HCC23.Steele C.L.Donaldson J.R.Paul D.Banes M.M.Arick T.Bridges S.M.Lawrence M.L.doi:10.1128/jb.05236-112011J. Bacteriol.1933679-3680NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]HCC23NAD_bind_Glutamyl_tRNA_reductGlutamyl-tRNA reductase, N-terminal domainNAD(P)-binding Rossmann-like DomainGlu_tRNA_reductase4pyrrol_synth_GluRdtase4pyrrol_synth_GluRdtase_dimer4pyrrol_synth_GluRdtase_NGluRdtase_CSGluRdtase_dimer_dom_sfGluRdtase_N_sfNAD(P)-bd_dom_sfShikm_DH/Glu-tRNA_RdtasehemAGLUTAMYL-TRNA REDUCTASE 1, CHLOROPLASTICGLUTAMYL-TRNA REDUCTASE 1, CHLOROPLASTICGlutR_dimerGlutR_NShikimate_DH4pyrrol_synth_GluRdtaseGlutamyl tRNA-reductase catalytic, N-terminal domainGlutamyl tRNA-reductase dimerization domainNAD(P)-binding Rossmann-fold domainsGLUTRGlutamyl-tRNA reductaseGluTR1.2.1.70hemALMHCC_1012Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).Homodimer.Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.Belongs to the glutamyl-tRNA reductase family.Glutamyl-tRNA reductase492521435Nucleophile504952109114116120189194Important for activity99substrate2009-03-031492523326573a0a48c354ce882878c0b0e688MFILTMGLNHHTAPIDIREKLVFKETEEEMALVTLQQEKSILENVIISTCNRTEIVAVVDQIHTGRYYLKRFMANWFQMDMEKIEPYLFFHEESEAVNHLYKVTAGLDSLVLGETQILGQVKHAFEIAKQTATTGTLLNKLFREVVTFAKKVHHHTKINENAVSVSYAAVEVAKKLYGSLDNKKIVLIGAGEMSELALQNLAGSGMTDITIINRTKTNAELLANQFQAKVGAYENMNEHLLLADIVLVSTSAAEPIIKQAAMQELMEQKASSMLVIDIGLPRNVEHDCSYIPNFHLYDIDDLAGVVTANSIERQQIVLELEDTIESEVRNFFEWEKQLGVVPVIRALREKALDMQEVTMTSLENKLPGLTEREYIQIGKHMKSIINQMLKQPISELKEMSVEEDATTSIEHFKRIFGLTKTDVTIIEKEQAETRStrue