B8DF20 (B8DF20_LISMH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 HAMAP MF_00108
EC=2.7.7.60 HAMAP MF_00108

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2 HAMAP MF_00108
MEP cytidylyltransferase 2 HAMAP MF_00108

Gene names

Name:	ispD EMBL ACK40742.1
Synonyms:	ispD2 HAMAP MF_00108
Ordered Locus Names:	LMHCC_2405

Organism

Listeria monocytogenes serotype 4a (strain HCC23) [Complete proteome] [HAMAP] EMBL ACK40742.1

Taxonomic identifier

552536 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108

Ontologies

Keywords
Biological process	Isoprene biosynthesis SAAS SAAS018294 HAMAP MF_00108
Molecular function	Nucleotidyltransferase SAAS SAAS018294 HAMAP MF_00108 EMBL ACK40742.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	15	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	22	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	153	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	209	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	B8DF20 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 45E7FC27FDDF3A17	FASTA	232	25,885
	10 20 30 40 50 60 MNYELVFLAA GQGKRMNAQK NKMWLELVGE PIFIHALRPF LADNRCSKVI VVCQEEERKH 70 80 90 100 110 120 VKELMSQLNV AESRIEIVKG GSERQYSVAA GLERCGTERV VLVHDGARPF ITVDIIDRLL 130 140 150 160 170 180 IGVKQSKAAI CAVKVKDTVK RVVNGVVQET VDRENLWQVQ TPQAFELPIL RKAHQLARKE 190 200 210 220 230 QFLGTDEASL VERLPCPVAI VQGSYYNIKL TTPEDMPLAK AILGELGGIA ND

References

[1]	"Genome sequence of lineage III Listeria monocytogenes strain HCC23." Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T., Bridges S.M., Lawrence M.L. J. Bacteriol. 193:3679-3680(2011) [PubMed: 21602330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HCC23.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001175 Genomic DNA. Translation: ACK40742.1.
RefSeq	YP_002351356.1. NC_011660.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8DF20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7078859.
GenomeReviews	Gene locus LMHCC_2405 in contig CP001175_GR.
KEGG	lmh:LMHCC_2405.
PATRIC	20319935. VBILisMon86872_2390.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	SKVIVVC.
ProtClustDB	PRK13385.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8DF20_LISMH

Accession

Primary (citable) accession number: B8DF20

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)