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B8DE74

- DAPA_LISMH

UniProt

B8DE74 - DAPA_LISMH

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Protein
4-hydroxy-tetrahydrodipicolinate synthase
Gene
dapA, LMHCC_1133
Organism
Listeria monocytogenes serotype 4a (strain HCC23)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA) By similarity.UniRule annotation

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei46 – 461Part of a proton relay during catalysis By similarity
Binding sitei47 – 471Pyruvate By similarity
Sitei110 – 1101Part of a proton relay during catalysis By similarity
Active sitei136 – 1361Proton donor/acceptor By similarity
Active sitei164 – 1641Schiff-base intermediate with substrate By similarity
Binding sitei206 – 2061Pyruvate; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
  2. amine-lyase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciLMON552536:GIW4-1169-MONOMER.
UniPathwayiUPA00034; UER00017.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthase (EC:4.3.3.7)
Short name:
HTPA synthase
Gene namesi
Name:dapA
Ordered Locus Names:LMHCC_1133
OrganismiListeria monocytogenes serotype 4a (strain HCC23)
Taxonomic identifieri552536 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000743: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2932934-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation
PRO_1000134870Add
BLAST

Interactioni

Subunit structurei

Homotetramer; dimer of dimers By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi552536.LMHCC_1133.

Structurei

3D structure databases

ProteinModelPortaliB8DE74.

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000173604.
KOiK01714.
OMAiEMEYIID.
OrthoDBiEOG6W7235.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00418. DapA.
InterProiIPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
TIGRFAMsiTIGR00674. dapA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8DE74-1 [UniParc]FASTAAdd to Basket

« Hide

MDLGKVITAM VTPIHPEKDK VCKKRIHHLV NHLIENGSDG LVIAGTTGES    50
PTLSHDEKIK LFRQVIETND GRAKLIAGTG SNNTAETIAF TKEVAELGGI 100
DAVLIVAPYY NKPNQDGLYA HFAAVAEASD LPVVIYNIPG RSVVNIEPET 150
IIRLAALPNI VGVKESSGNL DNISKIIAET SDDFQVYSGD DSLTLPILAV 200
GGNGVISVAS HVVGNEMQEM IQAFERGEVQ KAAQIHRELL PLMNGLFSVP 250
NPAPTKYLLN QQGISVGPVR LPLVDLNAEQ GTKLQAILEG LSK 293
Length:293
Mass (Da):31,348
Last modified:March 3, 2009 - v1
Checksum:iAC0143A92EA2A0DF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001175 Genomic DNA. Translation: ACK39481.1.
RefSeqiYP_002350095.1. NC_011660.1.

Genome annotation databases

EnsemblBacteriaiACK39481; ACK39481; LMHCC_1133.
GeneIDi7081080.
KEGGilmh:LMHCC_1133.
PATRICi20317381. VBILisMon86872_1121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001175 Genomic DNA. Translation: ACK39481.1 .
RefSeqi YP_002350095.1. NC_011660.1.

3D structure databases

ProteinModelPortali B8DE74.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 552536.LMHCC_1133.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK39481 ; ACK39481 ; LMHCC_1133 .
GeneIDi 7081080.
KEGGi lmh:LMHCC_1133.
PATRICi 20317381. VBILisMon86872_1121.

Phylogenomic databases

eggNOGi COG0329.
HOGENOMi HOG000173604.
KOi K01714.
OMAi EMEYIID.
OrthoDBi EOG6W7235.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00017 .
BioCyci LMON552536:GIW4-1169-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00418. DapA.
InterProi IPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
TIGRFAMsi TIGR00674. dapA. 1 hit.
PROSITEi PS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of lineage III Listeria monocytogenes strain HCC23."
    Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T., Bridges S.M., Lawrence M.L.
    J. Bacteriol. 193:3679-3680(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HCC23.

Entry informationi

Entry nameiDAPA_LISMH
AccessioniPrimary (citable) accession number: B8DE74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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