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Protein

Heme-degrading monooxygenase

Gene

isdG

Organism
Listeria monocytogenes serotype 4a (strain HCC23)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the biliverdin in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.UniRule annotation

Catalytic activityi

Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi6 – 61IronUniRule annotation
Sitei74 – 741Transition state stabilizerUniRule annotation
Metal bindingi84 – 841Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

  1. heme binding Source: UniProtKB-HAMAP
  2. heme oxygenase (decyclizing) activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. monooxygenase activity Source: UniProtKB-KW

GO - Biological processi

  1. heme catabolic process Source: UniProtKB-HAMAP
  2. iron assimilation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciLMON552536:GIW4-2191-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Heme-degrading monooxygenaseUniRule annotation (EC:1.14.99.3UniRule annotation)
Alternative name(s):
Heme oxygenaseUniRule annotation
Iron-regulated surface determinantUniRule annotation
Iron-responsive surface determinantUniRule annotation
Gene namesi
Name:isdGUniRule annotation
Ordered Locus Names:LMHCC_2158
OrganismiListeria monocytogenes serotype 4a (strain HCC23)
Taxonomic identifieri552536 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000743 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121121Heme-degrading monooxygenasePRO_1000165181Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi552536.LMHCC_2158.

Structurei

3D structure databases

ProteinModelPortaliB8DCJ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 101100ABMUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.UniRule annotation
Contains 1 ABM domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG2329.
HOGENOMiHOG000008026.
KOiK07145.
OMAiISTRWKE.
OrthoDBiEOG6GTZMS.

Family and domain databases

HAMAPiMF_01272. Heme_degrading_monooxygenase.
InterProiIPR007138. ABM-like.
IPR011008. Dimeric_a/b-barrel.
IPR023953. IsdG.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8DCJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIVTNTIKV EKGAAEHVIR QFTGANGDGH PTKDIAEVEG FLGFELWHSK
60 70 80 90 100
PEDKDYEEVV VTSKWESEEA QRNWVKSDSF KKAHGRTKDT REQREDRKGI
110 120
VGNAIARFEV VHVQNPVIVE K
Length:121
Mass (Da):13,785
Last modified:March 2, 2009 - v1
Checksum:i95674EA508DA2D49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001175 Genomic DNA. Translation: ACK40496.1.
RefSeqiYP_002351110.1. NC_011660.1.

Genome annotation databases

EnsemblBacteriaiACK40496; ACK40496; LMHCC_2158.
KEGGilmh:LMHCC_2158.
PATRICi20319444. VBILisMon86872_2145.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001175 Genomic DNA. Translation: ACK40496.1.
RefSeqiYP_002351110.1. NC_011660.1.

3D structure databases

ProteinModelPortaliB8DCJ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi552536.LMHCC_2158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK40496; ACK40496; LMHCC_2158.
KEGGilmh:LMHCC_2158.
PATRICi20319444. VBILisMon86872_2145.

Phylogenomic databases

eggNOGiCOG2329.
HOGENOMiHOG000008026.
KOiK07145.
OMAiISTRWKE.
OrthoDBiEOG6GTZMS.

Enzyme and pathway databases

BioCyciLMON552536:GIW4-2191-MONOMER.

Family and domain databases

HAMAPiMF_01272. Heme_degrading_monooxygenase.
InterProiIPR007138. ABM-like.
IPR011008. Dimeric_a/b-barrel.
IPR023953. IsdG.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of lineage III Listeria monocytogenes strain HCC23."
    Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T., Bridges S.M., Lawrence M.L.
    J. Bacteriol. 193:3679-3680(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HCC23.

Entry informationi

Entry nameiHDOX_LISMH
AccessioniPrimary (citable) accession number: B8DCJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2009
Last sequence update: March 2, 2009
Last modified: March 31, 2015
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.