B8DCC8 (B8DCC8_LISMH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 HAMAP MF_00108
EC=2.7.7.60 HAMAP MF_00108

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1 HAMAP MF_00108
MEP cytidylyltransferase 1 HAMAP MF_00108

Gene names

Name:	ispD1 HAMAP MF_00108
Ordered Locus Names:	LMHCC_1547

Organism

Listeria monocytogenes serotype 4a (strain HCC23) [Complete proteome] [HAMAP] EMBL ACK39891.1

Taxonomic identifier

552536 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294
Molecular function	Nucleotidyltransferase SAAS SAAS018294 HAMAP MF_00108 EMBL ACK39891.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	14	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	22	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	159	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	216	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	B8DCC8 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 6031EEB83214FD51	FASTA	237	26,782
	10 20 30 40 50 60 MIYAQILAGG KGTRMGNVSM PKQFLPLNGK PIIVHTVEKF ILNTRFDKIL ISSPKEWMNH 70 80 90 100 110 120 AEDNIKKYIS DDRIVVIEGG EDRNETIMNG IRFVEKTYGL TDDDIIVTHD AVRPFLTHRI 130 140 150 160 170 180 IEENIDAALE TGAVDTVIEA LDTIVESSNH EVITDIPVRD HMYQGQTPQS FNMKKVYSHY 190 200 210 220 230 QNLTPEKKQI LTDACKICLL AGDDVKLVKG EIFNIKITTP YDLKVANAII QERIAND

References

[1]	"Genome sequence of lineage III Listeria monocytogenes strain HCC23." Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T., Bridges S.M., Lawrence M.L. J. Bacteriol. 193:3679-3680(2011) [PubMed: 21602330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HCC23.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001175 Genomic DNA. Translation: ACK39891.1.
RefSeq	YP_002350505.1. NC_011660.1.
3D structure databases
ProteinModelPortal	B8DCC8.
ModBase	Search...
Protein-protein interaction databases
STRING	B8DCC8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7078602.
GenomeReviews	Gene locus LMHCC_1547 in contig CP001175_GR.
KEGG	lmh:LMHCC_1547.
PATRIC	20318207. VBILisMon86872_1533.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	GELFNIK.
ProtClustDB	PRK00155.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8DCC8_LISMH

Accession

Primary (citable) accession number: B8DCC8

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)