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B8DBR3

- DAPF_LISMH

UniProt

B8DBR3 - DAPF_LISMH

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Listeria monocytogenes serotype 4a (strain HCC23)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141SubstrateUniRule annotation
    Binding sitei53 – 531SubstrateUniRule annotation
    Binding sitei73 – 731SubstrateUniRule annotation
    Active sitei82 – 821Proton donor/acceptorUniRule annotation
    Binding sitei170 – 1701SubstrateUniRule annotation
    Sitei172 – 1721Important for catalytic activityUniRule annotation
    Binding sitei206 – 2061SubstrateUniRule annotation
    Sitei224 – 2241Important for catalytic activityUniRule annotation
    Active sitei233 – 2331Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciLMON552536:GIW4-559-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:LMHCC_0542
    OrganismiListeria monocytogenes serotype 4a (strain HCC23)
    Taxonomic identifieri552536 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000000743: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Diaminopimelate epimerasePRO_1000124419Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi82 ↔ 233

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi552536.LMHCC_0542.

    Structurei

    3D structure databases

    ProteinModelPortaliB8DBR3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni82 – 843Substrate bindingUniRule annotation
    Regioni224 – 2252Substrate bindingUniRule annotation
    Regioni234 – 2352Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiKFNDITT.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8DBR3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATIHFTKVH GSQNDFFLVD EEENLITEWS DEQRADFAIK LCDRKHSLGG    50
    ADGILYVTKS SEVGPIGQMR VVNSDGSIAS MCGNGLRTVA RYLLEKHALT 100
    EAKVETMKAI LDVKKATSLG FDIPTYQVEI SPVKFAAETL PMHVGVEKLF 150
    NQVIPELDTE LAFSAVSVPN PHLITFVDQS VLDSDKQEKL ASYLNSENPY 200
    FPDGVNVSFV KRLSDDAIYV RTFERGVGFT NACGTAMSAC SLIKKMLDND 250
    TFETPLNVYN DGGRVQVTAK EDAAGEISLQ LIGNATFVSK GSVRYENDIV 300
    TELTNEATAE QALYQALVKE VKEFLKTTE 329
    Length:329
    Mass (Da):36,199
    Last modified:March 3, 2009 - v1
    Checksum:iCAD952C4FCD29EDE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001175 Genomic DNA. Translation: ACK38899.1.
    RefSeqiYP_002349513.1. NC_011660.1.

    Genome annotation databases

    EnsemblBacteriaiACK38899; ACK38899; LMHCC_0542.
    GeneIDi7081484.
    KEGGilmh:LMHCC_0542.
    PATRICi20316155. VBILisMon86872_0537.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001175 Genomic DNA. Translation: ACK38899.1 .
    RefSeqi YP_002349513.1. NC_011660.1.

    3D structure databases

    ProteinModelPortali B8DBR3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 552536.LMHCC_0542.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK38899 ; ACK38899 ; LMHCC_0542 .
    GeneIDi 7081484.
    KEGGi lmh:LMHCC_0542.
    PATRICi 20316155. VBILisMon86872_0537.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi KFNDITT.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci LMON552536:GIW4-559-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of lineage III Listeria monocytogenes strain HCC23."
      Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T., Bridges S.M., Lawrence M.L.
      J. Bacteriol. 193:3679-3680(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HCC23.

    Entry informationi

    Entry nameiDAPF_LISMH
    AccessioniPrimary (citable) accession number: B8DBR3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3