B8DBP0 (NAMA_LISMH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH dehydrogenase
EC=1.6.99.1

Alternative name(s):
Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	LMHCC_0129

Organism

Listeria monocytogenes serotype 4a (strain HCC23) [Complete proteome] [HAMAP]

Taxonomic identifier

552536 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity. HAMAP MF_01614
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614
Cofactor	FMN By similarity. HAMAP MF_01614
Subunit structure	Homotetramer By similarity. HAMAP MF_01614
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	NADPH dehydrogenase HAMAP MF_01614		PRO_1000185865
Regions
	Nucleotide binding	22 – 26	5	FMN By similarity
Sites
	Binding site	27	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	214	1	FMN By similarity
	Binding site	307	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8DBP0 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 9231E7DC09C672B4	FASTA	338	37,060
	10 20 30 40 50 60 MSKLFSEYKL KDVTLKNRIV MSPMCMYSVE NKDGIATDFH FAHYVSRAAG GTGLVILEAT 70 80 90 100 110 120 AVQEVGRISE FDLGLWNDEQ VPALKRLVDG LHYHGAKAGI QLAHAGRKAV LPGEIVAPSA 130 140 150 160 170 180 IPFDEKSAKP VELTKEAIKE VVADFKRAAY RAKEAGFDVI EIHAAHGYLI HQFLSPITNR 190 200 210 220 230 240 REDNYGGPAG NRYKILSDII KAVKEVWDGP IIVRVSATDY AHGGLQLEDH IPFAKWMKAD 250 260 270 280 290 300 GVELIDVSTG GLVNVAPPVF PGYQVPFADE IRRGAGIATG ALGLITRGEQ AEEILCNERA 310 320 330 DLIIVGRELL RNPYFAKDAA EQLGETIEGP KQYSRAWK

References

[1]	"Genome sequence of lineage III Listeria monocytogenes strain HCC23." Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T., Bridges S.M., Lawrence M.L. J. Bacteriol. 193:3679-3680(2011) [PubMed: 21602330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HCC23.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001175 Genomic DNA. Translation: ACK38491.1.
RefSeq	YP_002349105.1. NC_011660.1.
3D structure databases
ProteinModelPortal	B8DBP0.
ModBase	Search...
Protein-protein interaction databases
STRING	B8DBP0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7079326.
GenomeReviews	Gene locus LMHCC_0129 in contig CP001175_GR.
KEGG	lmh:LMHCC_0129.
PATRIC	20315280. VBILisMon86872_0125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG583461.
OMA	WVEDGWN.
ProtClustDB	PRK13523.
Family and domain databases
HAMAP	MF_01614. NamA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR023663. NADPH_DH. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00540.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_LISMH

Accession

Primary (citable) accession number: B8DBP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program