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B8D9X3

- TRMB_BUCA5

UniProt

B8D9X3 - TRMB_BUCA5

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691S-adenosyl-L-methionineUniRule annotation
    Binding sitei94 – 941S-adenosyl-L-methionineUniRule annotation
    Binding sitei121 – 1211S-adenosyl-L-methionineUniRule annotation
    Active sitei144 – 1441By similarity
    Binding sitei144 – 1441S-adenosyl-L-methionineUniRule annotation
    Binding sitei148 – 1481SubstrateUniRule annotation
    Binding sitei180 – 1801SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBAPH563178:GHDF-544-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:BUAP5A_544
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A)
    Taxonomic identifieri563178 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000006904: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239tRNA (guanine-N(7)-)-methyltransferasePRO_1000149648Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi107806.BU551.

    Structurei

    3D structure databases

    ProteinModelPortaliB8D9X3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni217 – 2204Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiFATDWEN.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8D9X3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNNIITPRY NLEGVFLRQI HSFVCRKGRT TTSQLSAIKK YWSLIGVDFQ    50
    LNALNFSSIF KHRAPIILEI GFGSGESLVK TAMNFPEKNF LGIEVYKSGI 100
    GSCLHYASSY QIQNLRIIYY DATEVMYNMI PDDTLSKVQI FFPDPWHKKR 150
    HHKRRLLKNI FLKIITKKLI IDGILHIATD SESYAFYILD EIKDIKNYKN 200
    LSEKNNFVKR PVSRIITKFE KKGLLQGKKI FDLMFQLKK 239
    Length:239
    Mass (Da):27,935
    Last modified:March 3, 2009 - v1
    Checksum:iD9566620ECC8405F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001161 Genomic DNA. Translation: ACL30894.1.
    RefSeqiYP_002468282.1. NC_011833.1.

    Genome annotation databases

    EnsemblBacteriaiACL30894; ACL30894; BUAP5A_544.
    GeneIDi7262306.
    KEGGibap:BUAP5A_544.
    PATRICi21243355. VBIBucAph51993_0540.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001161 Genomic DNA. Translation: ACL30894.1 .
    RefSeqi YP_002468282.1. NC_011833.1.

    3D structure databases

    ProteinModelPortali B8D9X3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 107806.BU551.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL30894 ; ACL30894 ; BUAP5A_544 .
    GeneIDi 7262306.
    KEGGi bap:BUAP5A_544.
    PATRICi 21243355. VBIBucAph51993_0540.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi FATDWEN.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci BAPH563178:GHDF-544-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria."
      Moran N.A., McLaughlin H.J., Sorek R.
      Science 323:379-382(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 5A.

    Entry informationi

    Entry nameiTRMB_BUCA5
    AccessioniPrimary (citable) accession number: B8D9X3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3