B8D9K2 (CYSD_BUCA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	BUAP5A_417

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A) [Complete proteome] [HAMAP]

Taxonomic identifier

563178 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_1000117937

Sequences

	Sequence		Length	Mass (Da)
	B8D9K2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 11B1E4F48F8A2ADA	FASTA	302	35,514
	10 20 30 40 50 60 MFKKNTTHLR QLESESIYIM REVMSDFQNP VMLYSIGKDS SVMLHLAKKS FYPGTIPFPL 70 80 90 100 110 120 LHIDTGWKFK EMYIFRDHIA STSNIELIVH SHSKGKLLGL NPFENGGSKY TDIMKTEGLK 130 140 150 160 170 180 EAINKYNFDA AFGGARRDEE KSRSKERIYS FRDSLHQWDP KKQRPELWWN YNGQINKGEN 190 200 210 220 230 240 IRVFPLSNWT ELDIWQYIFL EKIEIVPLYF AAKRPVLERN GVLTVIDDSR MKIKSNEVIK 250 260 270 280 290 300 EKMVRFRTLG CWPLTNAIES EARNVEDIIK ETLIVKTSER TGRAIDYDQK SSMEFKKRQG YF

References

[1]	"The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria." Moran N.A., McLaughlin H.J., Sorek R. Science 323:379-382(2009) [PubMed: 19150844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 5A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001161 Genomic DNA. Translation: ACL30773.1.
RefSeq	YP_002468161.1. NC_011833.1.
3D structure databases
ProteinModelPortal	B8D9K2.
SMR	B8D9K2. Positions 7-212.
ModBase	Search...
Protein-protein interaction databases
STRING	B8D9K2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000001154; EBBUCP00000001109; EBBUCG00000001154.
GeneID	7262435.
GenomeReviews	Gene locus BUAP5A_417 in contig CP001161_GR.
KEGG	bap:BUAP5A_417.
PATRIC	21243099. VBIBucAph51993_0418.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008041.
OMA	YTQVMKT.
ProtClustDB	PRK05253.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_BUCA5

Accession

Primary (citable) accession number: B8D9K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program