B8D9J8 (B8D9J8_BUCA5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108 RuleBase RU003490
EC=2.7.7.60 HAMAP MF_00108 RuleBase RU003490

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ygbP EMBL ACL30769.1
Synonyms:	ispD HAMAP MF_00108
Ordered Locus Names:	BUAP5A_413

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A) [Complete proteome] [HAMAP]

Taxonomic identifier

563178 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004138
Subunit structure	Homodimer By similarity. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108 RuleBase RU004393

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393
Molecular function	Nucleotidyltransferase HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393 EMBL ACL30769.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	23	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	30	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	160	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	216	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	B8D9J8 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 3E82D5CF35BCB908	FASTA	237	26,921
	10 20 30 40 50 60 MILVNLFEPK IIAIVPAAGI GSRMKIDVPK QYIKIQNRTI LEHTLTTLLL HPNIVQIIVS 70 80 90 100 110 120 LNKKDNYFHK LSISSNFRII SVVGGEKRIN SVLSGLIVVK NVDWVIVHDA VRPCLSYKDL 130 140 150 160 170 180 EKLISIIKKN PVGAILARPV SDTIKYSNLK QKKAVYTVYR KNLWHALTPQ LFQVKLLKNC 190 200 210 220 230 LKKIIKDQIS VTDEASALEY CGYNPLLVLG SCRNIKITWP EDLVLANFYL KNHIIDK

References

[1]	"The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria." Moran N.A., McLaughlin H.J., Sorek R. Science 323:379-382(2009) [PubMed: 19150844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 5A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001161 Genomic DNA. Translation: ACL30769.1.
RefSeq	YP_002468157.1. NC_011833.1.
3D structure databases
ProteinModelPortal	B8D9J8.
ModBase	Search...
Protein-protein interaction databases
STRING	B8D9J8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000860; EBBUCP00000000815; EBBUCG00000000860.
GeneID	7262431.
GenomeReviews	Gene locus BUAP5A_413 in contig CP001161_GR.
KEGG	bap:BUAP5A_413.
PATRIC	21243091. VBIBucAph51993_0414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007877.
OMA	FPQGAIL.
ProtClustDB	PRK00155.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8D9J8_BUCA5

Accession

Primary (citable) accession number: B8D9J8

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)