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B8D983

- BIOB_BUCA5

UniProt

B8D983 - BIOB_BUCA5

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Protein

Biotin synthase

Gene

bioB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi95 – 951Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi126 – 1261Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi186 – 1861Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi258 – 2581Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBAPH563178:GHDF-287-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:BUAP5A_286
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A)
Taxonomic identifieri563178 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000006904: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Biotin synthasePRO_0000381256Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi107806.BU291.

Structurei

3D structure databases

ProteinModelPortaliB8D983.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B8D983-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKKWTLEET KMLFKKPFFN LMFQAQEEHR KNFDPNTIQI STLLSIKTGS
60 70 80 90 100
CPEDCKYCPQ SSRYKTGLKK EPLLEIEQIL SAAKKAKDSG SSRFCMGAAW
110 120 130 140 150
KNPKEKDMPY LEKIIKKIKE MGMETCMTLG TLNSTQAKKL ADAGLDFYNH
160 170 180 190 200
NLDTSKNFYS NIITTRTYQE RLHTLHAVRS SGMKVCSGGI IGLGEKKQDR
210 220 230 240 250
IELLIELSNL SIQPESVPIN MLVKIPGTPM ADNKDVEPFD FIRIIAVARI
260 270 280 290 300
MMPKSYIRLS AGRHKMNDQT QAMCFMAGAN SIFYGCKLLT SDNPEEKHDL
310 320 330 340
ELFKKLDLIP ENKTQTLLKE DEYKTIIKSS KIKKDQYYNA AII
Length:343
Mass (Da):39,167
Last modified:March 3, 2009 - v1
Checksum:i4402C35CDDAABAE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001161 Genomic DNA. Translation: ACL30654.1.
RefSeqiYP_002468042.1. NC_011833.1.

Genome annotation databases

EnsemblBacteriaiACL30654; ACL30654; BUAP5A_286.
GeneIDi7262295.
KEGGibap:BUAP5A_286.
PATRICi21242821. VBIBucAph51993_0288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001161 Genomic DNA. Translation: ACL30654.1 .
RefSeqi YP_002468042.1. NC_011833.1.

3D structure databases

ProteinModelPortali B8D983.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 107806.BU291.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL30654 ; ACL30654 ; BUAP5A_286 .
GeneIDi 7262295.
KEGGi bap:BUAP5A_286.
PATRICi 21242821. VBIBucAph51993_0288.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci BAPH563178:GHDF-287-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria."
    Moran N.A., McLaughlin H.J., Sorek R.
    Science 323:379-382(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 5A.

Entry informationi

Entry nameiBIOB_BUCA5
AccessioniPrimary (citable) accession number: B8D983
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3