B8D7U5 (SYQ_BUCAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	BUAPTUC7_409

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7) [Complete proteome] [HAMAP]

Taxonomic identifier

561501 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_1000199092
Regions
	Motif	35 – 45	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	269 – 273	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	272	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8D7U5 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 1564C165FB5B5D84	FASTA	571	67,834
	10 20 30 40 50 60 MDTKKEIKNN NFICQIINKD LNENKNLSFY TRFPPEPNGY LHIGHAKSIC LNFELASLYK 70 80 90 100 110 120 GRCNLRFDDT NPLKENIKYI KSIKHDINWL GYKWHGNVRY ASEYFLKLYQ YAQELIKKGL 130 140 150 160 170 180 AYVDHLTKEQ IREYRGTLNT PGKNSPYRNR TIQENIELFE KMKKGDFSEG EACLRAKINM 190 200 210 220 230 240 SSSSIIMRDP VLYRIIFIKH HQTQNKWCIY PMYDFAHCLS DSIEGITHSL CTLEFQDNKF 250 260 270 280 290 300 LYNWILKNTS VKHYPKQYEF SRLNLEFSIL SKRKIKILID KNIIEGWDDP RIPTLSALRR 310 320 330 340 350 360 KGYTPSSIKN FCQKIGVTKQ NNLIEFSMLE HCIRKELNQT AIRTMAILDP IKIFLYNLDS 370 380 390 400 410 420 NYKEEFIVPN HPNNPEMGTH KIIFTNTIYI DRSDFKEKYD KKYKRLKLGE KIRLRYSYII 430 440 450 460 470 480 HAEKIEKDEY GNISNIICYC DLNTLGRKPK DNKNPAVIHW ISEKNTLSAE FKLYDQLFNI 490 500 510 520 530 540 KNPEQQENFL LYINSKSLIK KFGFIEKKIG EEIQKKISNN NIEIFFQFER IGYFCIDFID 550 560 570 SKKNQLVFNR TVGLRDTWDS KKIKTKNITN N

References

[1]	"The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria." Moran N.A., McLaughlin H.J., Sorek R. Science 323:379-382(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuc7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001158 Genomic DNA. Translation: ACL30210.1.
RefSeq	YP_002468705.1. NC_011834.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	561501.BUAPTUC7_409.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL30210; ACL30210; BUAPTUC7_409.
GeneID	7263024.
KEGG	bau:BUAPTUC7_409.
PATRIC	21248923. VBIBucAph26317_0407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
KO	K01886.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	BAPH561501:GHRN-406-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_BUCAT

Accession

Primary (citable) accession number: B8D7U5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program