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B8D7R0

- PNP_BUCAT

UniProt

B8D7R0 - PNP_BUCAT

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi486 – 4861MagnesiumUniRule annotation
    Metal bindingi492 – 4921MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciBAPH561501:GHRN-365-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:BUAPTUC7_367
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7)
    Taxonomic identifieri561501 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000002455: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 707707Polyribonucleotide nucleotidyltransferasePRO_1000185727Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

    Protein-protein interaction databases

    STRINGi561501.BUAPTUC7_367.

    Structurei

    3D structure databases

    ProteinModelPortaliB8D7R0.
    SMRiB8D7R0. Positions 617-691.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini553 – 61260KHUniRule annotationAdd
    BLAST
    Domaini622 – 69069S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8D7R0-1 [UniParc]FASTAAdd to Basket

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    MLNPIVRKFQ YGQHTITLET GVIARQANAA VMASMDETAV FVTVVGQKKI    50
    HTGQKFFPLT VNYQERTYAA GRIPGGFFRR EGRPSENEIL TARLIDRPLR 100
    PLFPKKFLNE IQIIATVVSV NPQINPDIIS IIGASAALSL SGIPFYGPVG 150
    AARVGYINNQ YILNPISDDM KNSSLDLVVS GTQNAILMVE AESKILSEEK 200
    ILGAIIFGHQ QQQVVINNIR SLSNEASKLP WVISYPETNK TLELKIINSF 250
    EKNISDAYVI FNKQDRIEKL NSIKENIIKL FLDENSNIDT LEIEDIFQKI 300
    EKKVVRKRIL SNQTRIDGRE KDMIRALDVR TGILPRTHGS ALFTRGETQS 350
    LVSVTLGTSR DAQNLDELLG DRIDNFLFHY NFPPYSVGEI GMVGSPKRRE 400
    IGHGRLAKRS LLAVMPTLEN FPYTIRVVSE ITESNGSSSM ASVCGASLAL 450
    MDAGVPIKSA VAGISMGLVK EGNQHVLLSD ILGDEDHLGD MDFKVAGTEE 500
    GITALQMDMK IEGITNEIIH SALNEARLAR LHILNVMNQA LNESRSEISE 550
    FAPRIHIIKI NPEKIKDVIG KGGSVIRMLT EETGTIIEIE DDGTVKISST 600
    VKEKAKNAIR RIKEITAEIE VGRIYSGKVT RIVDFGAFVS IGLGKEGLVH 650
    ISQISDKRVD KVSNHLKIDQ IISVKVLEID RQGRLRLSIK EIDSSILSNK 700
    SINNSII 707
    Length:707
    Mass (Da):78,234
    Last modified:March 3, 2009 - v1
    Checksum:i75FA1BBE08105B7A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001158 Genomic DNA. Translation: ACL30175.1.
    RefSeqiYP_002468670.1. NC_011834.1.

    Genome annotation databases

    EnsemblBacteriaiACL30175; ACL30175; BUAPTUC7_367.
    GeneIDi7262982.
    KEGGibau:BUAPTUC7_367.
    PATRICi21248837. VBIBucAph26317_0370.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001158 Genomic DNA. Translation: ACL30175.1 .
    RefSeqi YP_002468670.1. NC_011834.1.

    3D structure databases

    ProteinModelPortali B8D7R0.
    SMRi B8D7R0. Positions 617-691.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 561501.BUAPTUC7_367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL30175 ; ACL30175 ; BUAPTUC7_367 .
    GeneIDi 7262982.
    KEGGi bau:BUAPTUC7_367.
    PATRICi 21248837. VBIBucAph26317_0370.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci BAPH561501:GHRN-365-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria."
      Moran N.A., McLaughlin H.J., Sorek R.
      Science 323:379-382(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuc7.

    Entry informationi

    Entry nameiPNP_BUCAT
    AccessioniPrimary (citable) accession number: B8D7R0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3