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B8D754

- SYI_BUCAT

UniProt

B8D754 - SYI_BUCAT

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
Binding sitei607 – 6071ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH561501:GHRN-146-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BUAPTUC7_148
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7)
Taxonomic identifieri561501 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000002455: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000189134Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi561501.BUAPTUC7_148.

Structurei

3D structure databases

ProteinModelPortaliB8D754.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi604 – 6085"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8D754 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDYKDTLNL PKTLFSMRGN LSKKEPNILK SWNENNLYKL IRKKNQEKKI
60 70 80 90 100
FFLHDGPPYA NGNIHIGHAV NKILKDIIIK SKNMSGFDAP YIPSWDCHGL
110 120 130 140 150
PIEQKVEEKI KSNQGEISTT EFQEKCRKYA QDQVEKQKKD FIRLGVIGDW
160 170 180 190 200
DNPHLTMNFK NEANIIKTLS KIVQKKHLYQ DFKPIHWCLK CASSLSEAEI
210 220 230 240 250
EYSKKKSDSI IVGFKFKYRS IIEKLFDFQI SNKKEIHLLI WTTTPWTLPS
260 270 280 290 300
SKAISIHPDF QYQLIETERC YLIIAKELVE KTLNTLKIKK SIIRNYVKGR
310 320 330 340 350
FLEKMICLHP FLKNIDLPVI LGKHVTLESG TGAVHTAPDH GLEDYIISQK
360 370 380 390 400
YNIKTSNIVN FKGEYISNTH DKLDGVNVLE ANSIIIELLI KNNTFFHHES
410 420 430 440 450
LIHSYPHCWR HKSPVIYRAT PQWFIDIDQK QLRIKLLQEI KKVRWIPEWG
460 470 480 490 500
ESRIGEMIKK RPDWCISRQR KWGVPMSIFI HKNTRKIHPN TFVFMKKIAK
510 520 530 540 550
KVELEGLQVW WNIDSKEILG EEYQSYEKIL DILDVWFESG NTHTTINYKN
560 570 580 590 600
KNYTKKNADM FLEGSDQHRG WFMSSLIIST LISEKKPYSE VLTHGFVVDG
610 620 630 640 650
KGQKMSKSIG NTISPNEIVD TLGADILRLW VASSNYSNDI SISNEILKSS
660 670 680 690 700
SDIYRRIRNT ARFMLANISD FDPKKNIISK ENMVLLDKWA IGQTKIVQEE
710 720 730 740 750
IIQHYNNYNF HAVIQRLMYF CSIEMGSFYL DIIKDRQYTL KKHSQERRSS
760 770 780 790 800
QTAIYYIINS LVRWIAPILS FTADEIWSYL PENNSQYVFM EEWFDKLFYL
810 820 830 840 850
DQDDLFNYQF WNEIITIKHE INKFLEEAIQ NKTINNSLET SIILYVSHEL
860 870 880 890 900
SNKLKILEQE TKFIFLTSDI QIKLYDTAPK NAKKSKIVPY LKVSLEKIKG
910 920 930 940
KKCPRCWHYF NFTKKNIKNS DICNRCILNT IGNGEKRIFI
Length:940
Mass (Da):110,485
Last modified:March 3, 2009 - v1
Checksum:iD5CDFBFFA98F2FB2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001158 Genomic DNA. Translation: ACL29969.1.
RefSeqiYP_002468464.1. NC_011834.1.

Genome annotation databases

EnsemblBacteriaiACL29969; ACL29969; BUAPTUC7_148.
GeneIDi7262814.
KEGGibau:BUAPTUC7_148.
PATRICi21248369. VBIBucAph26317_0143.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001158 Genomic DNA. Translation: ACL29969.1 .
RefSeqi YP_002468464.1. NC_011834.1.

3D structure databases

ProteinModelPortali B8D754.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561501.BUAPTUC7_148.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL29969 ; ACL29969 ; BUAPTUC7_148 .
GeneIDi 7262814.
KEGGi bau:BUAPTUC7_148.
PATRICi 21248369. VBIBucAph26317_0143.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BAPH561501:GHRN-146-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria."
    Moran N.A., McLaughlin H.J., Sorek R.
    Science 323:379-382(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuc7.

Entry informationi

Entry nameiSYI_BUCAT
AccessioniPrimary (citable) accession number: B8D754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3