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Protein

Argininosuccinate synthase

Gene

argG

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei91 – 911CitrullineUniRule annotation
Binding sitei121 – 1211ATP; via amide nitrogenUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei127 – 1271AspartateUniRule annotation
Binding sitei127 – 1271CitrullineUniRule annotation
Binding sitei128 – 1281AspartateUniRule annotation
Binding sitei131 – 1311CitrullineUniRule annotation
Binding sitei180 – 1801CitrullineUniRule annotation
Binding sitei189 – 1891CitrullineUniRule annotation
Binding sitei265 – 2651CitrullineUniRule annotation
Binding sitei277 – 2771CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 209ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH561501:GHRN-49-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:BUAPTUC7_050
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7)
Taxonomic identifieri561501 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Argininosuccinate synthasePRO_1000191886Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB8D6W2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8D6W2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRKKNNKVV LAYSGGLDTS AIIPWLKENY NFEVVAFVAD IGQSKKDLNG
60 70 80 90 100
IEKKSLESGA SSCHVFDLKE EFIENYVYPV LKTGALYEGS YLLGTAMARP
110 120 130 140 150
IIAKKQVELA LNIGANSLCH GATGKGNDQV RFEMAYAALA PNLNVIAPWR
160 170 180 190 200
EWNLNSRESL LKYLDKKNIS TTATLEKIYS KDENSWHIST EGGLLENPWN
210 220 230 240 250
QSNEDCWSWT VNPEDAPEKP EYVSLQLKEG CVVSVNNQKL NPLKCVEELN
260 270 280 290 300
SLGAKHGIGR IDIIENRLIG MKSRGCYETP GGTIIMTAIK AIEQLVLDRE
310 320 330 340 350
SFRWREKIGL EMSSIVYDGR WFSPIRKSLQ AAADSLSLEI TGEVILKLYK
360 370 380 390 400
GSVTAVQKKS PNSLYSEEYA TFGEDKVYKQ SDADGFIRLF SLSSKIRAQN

MLK
Length:403
Mass (Da):45,073
Last modified:March 3, 2009 - v1
Checksum:iF23B7024C8BDB147
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001158 Genomic DNA. Translation: ACL29877.1.
RefSeqiWP_009874007.1. NC_011834.1.

Genome annotation databases

EnsemblBacteriaiACL29877; ACL29877; BUAPTUC7_050.
KEGGibau:BUAPTUC7_050.
PATRICi21248165. VBIBucAph26317_0045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001158 Genomic DNA. Translation: ACL29877.1.
RefSeqiWP_009874007.1. NC_011834.1.

3D structure databases

ProteinModelPortaliB8D6W2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL29877; ACL29877; BUAPTUC7_050.
KEGGibau:BUAPTUC7_050.
PATRICi21248165. VBIBucAph26317_0045.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciBAPH561501:GHRN-49-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria."
    Moran N.A., McLaughlin H.J., Sorek R.
    Science 323:379-382(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuc7.

Entry informationi

Entry nameiASSY_BUCAT
AccessioniPrimary (citable) accession number: B8D6W2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: July 22, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.