B8D6T1 (DNAA_BUCAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	BUAPTUC7_012

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7) [Complete proteome] [HAMAP]

Taxonomic identifier

561501 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000189786
Regions
	Nucleotide binding	159 – 166	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B8D6T1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 1F5299F4C2751213	FASTA	454	52,931
	10 20 30 40 50 60 MSLCLWKQCL DRLQDELPNT EFSMWIRSLK AKLNNNILEI YAPNKFVLEW VKDKYLNHLK 70 80 90 100 110 120 KILQDYCGTN SPLIKFEIYQ IYKENKLKKN IENNNNNKNE KLIWSNIPKF KNLSYRSNIN 130 140 150 160 170 180 KRYNFQNFVE GKSNQLARSA AFQAARNPGN SYNPLFLYGA TGLGKTHLLH AIGNEILSYK 190 200 210 220 230 240 YDIKIIFMNS ECFVQDMVKA LKNNAIEKFK LYYRSVDALL IDDIQFFAHK ERSQEEFFHT 250 260 270 280 290 300 FNTLIEGNQQ IILTSDRYPK EINGVEDRLK SRFSWGLTIA IDPPEIETRV AILIKKADQN 310 320 330 340 350 360 NVILSNEVAF FIAKHLRSNV RELEGALNRV ILNSRFTHRA ITVDFAREAL QDILAVQEKI 370 380 390 400 410 420 ITIDNIQKTV AKYYKIKVSD LLSKKRSRSI ARPRQMAMAM AKKLTNHSLP EIGEAFSGRD 430 440 450 HTTVLHACCK IEQLRKENHD IKKDFLNLIR TLSK

References

[1]	"The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria." Moran N.A., McLaughlin H.J., Sorek R. Science 323:379-382(2009) [PubMed: 19150844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuc7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001158 Genomic DNA. Translation: ACL29846.1.
RefSeq	YP_002468341.1. NC_011834.1.
3D structure databases
ProteinModelPortal	B8D6T1.
SMR	B8D6T1. Positions 2-78, 361-453.
ModBase	Search...
Protein-protein interaction databases
STRING	B8D6T1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002127; EBBUCP00000001997; EBBUCG00000002127.
GeneID	7263358.
GenomeReviews	Gene locus BUAPTUC7_012 in contig CP001158_GR.
KEGG	bau:BUAPTUC7_012.
PATRIC	21248089. VBIBucAph26317_0012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008033.
HOGENOM	HBG695666.
OMA	DEVAFLI.
ProtClustDB	PRK00149.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_BUCAT

Accession

Primary (citable) accession number: B8D6T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program