Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Desulfurococcus kamchatkensis (strain 1221n / DSM 18924)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei167Proton acceptorUniRule annotation1
Binding sitei169SubstrateUniRule annotation1
Metal bindingi193Magnesium; via carbamate groupUniRule annotation1
Metal bindingi195MagnesiumUniRule annotation1
Metal bindingi196MagnesiumUniRule annotation1
Active sitei285Proton acceptorUniRule annotation1
Binding sitei286SubstrateUniRule annotation1
Binding sitei318SubstrateUniRule annotation1
Sitei326Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:DKAM_1140Imported
OrganismiDesulfurococcus kamchatkensis (strain 1221n / DSM 18924)Imported
Taxonomic identifieri490899 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeDesulfurococcus
Proteomesi
  • UP000006903 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei193N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi490899.DKAM_1140.

Structurei

3D structure databases

ProteinModelPortaliB8D5T5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 136RuBisCO_large_NInterPro annotationAdd BLAST122
Domaini147 – 442RuBisCO_largeInterPro annotationAdd BLAST296

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni371 – 373Substrate bindingUniRule annotation3
Regioni393 – 396Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

B8D5T5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLMSGKFEF ETYHEYIDKN YTPDPSNDVI AVYRVKPAQG FTIEDAAGGV
60 70 80 90 100
AAESSTGTWT SLYNWYDVGR VRRLSGKAYY FKDLDDGSWI VKIAYPVELF
110 120 130 140 150
EEGNIPGLLA SIAGNIFGMK RVEGLRLEDI YLPKKFLESF KGPSKGLNGV
160 170 180 190 200
REIFGVKDRP IVGTVPKPKE GYSPEEVEKL ALELLSGGLD YIKDDENLTS
210 220 230 240 250
PSFCRFEARA KAIMKVIDKV EKETGERKVW FANITSDIRE MEKRLRLVAD
260 270 280 290 300
YGNPYIMVDV VITGWSALTY IRDLAEEYGL AIHGHRAMHA AFTRNPYHGI
310 320 330 340 350
SMYVLAKLYR IIGIDQLHIG TAGAGKLEGG KLDVIRYAKI LREIHFKPDP
360 370 380 390 400
DDMYHLEQPM HHIKPAMPVS SGGLHPGNLP PVIEALGTNL VLQIGGGVIG
410 420 430 440
HPDGPRAGAL AVRQALEAIM NNIPLDEYAK THRELARALE KWGFAKPI
Length:448
Mass (Da):49,948
Last modified:March 3, 2009 - v1
Checksum:i341A7EDE5549F78B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001140 Genomic DNA. Translation: ACL11466.1.

Genome annotation databases

EnsemblBacteriaiACL11466; ACL11466; DKAM_1140.
KEGGidka:DKAM_1140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001140 Genomic DNA. Translation: ACL11466.1.

3D structure databases

ProteinModelPortaliB8D5T5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi490899.DKAM_1140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL11466; ACL11466; DKAM_1140.
KEGGidka:DKAM_1140.

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB8D5T5_DESK1
AccessioniPrimary (citable) accession number: B8D5T5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: November 30, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.