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B8D5I3 (B8D5I3_DESK1) Unreviewed, UniProtKB/TrEMBL

Last modified November 16, 2011. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Inorganic pyrophosphatase HAMAP MF_00209
EC=3.6.1.1 HAMAP MF_00209
Alternative name(s):
Pyrophosphate phospho-hydrolase HAMAP MF_00209
Gene names
Name:ppa HAMAP MF_00209
Ordered Locus Names:DKAM_1038
OrganismDesulfurococcus kamchatkensis (strain 1221n / DSM 18924) [Complete proteome] [HAMAP]
Taxonomic identifier490899 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Desulfurococcus

Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP MF_00209

Cofactor

Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity. HAMAP MF_00209

Subcellular location

Cytoplasm By similarity HAMAP MF_00209.

Sequence similarities

Belongs to the PPase family. HAMAP MF_00209

Ontologies

Keywords
   Cellular componentCytoplasm HAMAP MF_00209
   LigandMagnesium HAMAP MF_00209
Metal-binding HAMAP MF_00209
   Molecular functionHydrolase HAMAP MF_00209
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processphosphate-containing compound metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioninorganic diphosphatase activity

Inferred from electronic annotation. Source: HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding661Magnesium 1 By similarity HAMAP MF_00209
Metal binding711Magnesium 1 By similarity HAMAP MF_00209
Metal binding711Magnesium 2 By similarity HAMAP MF_00209
Metal binding1031Magnesium 1 By similarity HAMAP MF_00209

Sequences

Sequence LengthMass (Da)Tools
B8D5I3 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 80E275F529AA5FF7

FASTA17519,612
        10         20         30         40         50         60 
MSQYSKLGPG SKAPEEVYVV IEIPVNSGVK YELDKETGVL MVDRILYTSM VYPFNYGFIP 

        70         80         90        100        110        120 
GTLEEDGDPV DVLVVSSEPL IPGSVIKVRP IGVLETEDEK GRDAKILAVP VDKVDPRYSN 

       130        140        150        160        170 
IRDVGDLPEA VKQRIKHFFE HYKELEPGKW VKVVGWKGRE EALKRINDAI NRAKG

« Hide

References

[1]"Complete genome sequence of the anaerobic, protein-degrading hyperthermophilic crenarchaeon Desulfurococcus kamchatkensis."
Ravin N.V., Mardanov A.V., Beletsky A.V., Kublanov I.V., Kolganova T.V., Lebedinsky A.V., Chernyh N.A., Bonch-Osmolovskaya E.A., Skryabin K.G.
J. Bacteriol. 191:2371-2379(2009) [PubMed: 19114480] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001140 Genomic DNA. Translation: ACL11364.1.
RefSeqYP_002428731.1. NC_011766.1.

3D structure databases

ProteinModelPortalB8D5I3.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8D5I3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7171150.
GenomeReviewsGene locus DKAM_1038 in contig CP001140_GR.
KEGGdka:DKAM_1038.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG529150.
OMAPNDFNVI.
ProtClustDBCLSK965756.

Family and domain databases

HAMAPMF_00209. Inorganic_PPase.
[Tree]
InterProIPR008162. Pyrophosphatase.
[Graphical view]
Gene3DG3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KOK01507.
PANTHERPTHR10286. Pyrophosphatase. 1 hit.
PfamPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMSSF50324. Pyrophosphatase. 1 hit.
PROSITEPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB8D5I3_DESK1
AccessionPrimary (citable) accession number: B8D5I3
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: November 16, 2011
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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