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B8D417 (B8D417_DESK1) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420
EC=6.3.5.3 HAMAP MF_00420
Alternative name(s):
Phosphoribosylformylglycinamidine synthase II HAMAP MF_00420
Gene names
Name:purL HAMAP MF_00420
Ordered Locus Names:DKAM_0522
OrganismDesulfurococcus kamchatkensis (strain 1221n / DSM 18924) [Complete proteome] [HAMAP]
Taxonomic identifier490899 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Desulfurococcus

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420

Subunit structure

Heterodimer of two subunits, PurQ and PurL By similarity. HAMAP MF_00420

Subcellular location

Cytoplasm By similarity HAMAP MF_00420.

Sequence similarities

Belongs to the FGAMS family. HAMAP MF_00420

Ontologies

Keywords
   Biological processPurine biosynthesis HAMAP MF_00420
   Cellular componentCytoplasm HAMAP MF_00420
   LigandATP-binding HAMAP MF_00420
Nucleotide-binding
   Molecular functionLigase HAMAP MF_00420
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequences

Sequence LengthMass (Da)Tools
B8D417 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 4DE6AAAA2CD5490D

FASTA73180,526
        10         20         30         40         50         60 
MVKMPLSVEE IDEIRDILKR EPTREELVMF EAHWSEHCSY KSSRRLLRLL PNKGNHVVLG 

        70         80         90        100        110        120 
PGRDAPAVEV FPGILAVFKI ESHNHPSAVD PYNGAATGIG GIIRDILTLG AKPIALLDLL 

       130        140        150        160        170        180 
YMGNPFNPHA KWLIRNIVKG ISDYGNRVGV PVVAGDTWFD ESFNKQPLVN VACLGIVEKN 

       190        200        210        220        230        240 
KAVLSKPRPG DLVVIIGNST GRDGLLGSSF ASAPLKDNMV EDIGAVQIGD ALMEKMLIDV 

       250        260        270        280        290        300 
VNEVVEKKLV DYIKDLGGGG LTTAISEAAG DYDLGVEIYL DRLHLRMDLE PYEILASESQ 

       310        320        330        340        350        360 
ERMLLAVPRE NFNELIGILE KYEVPYSVIG IFNDSGFIRV YYKGRIIAEV PSKAMARPRA 

       370        380        390        400        410        420 
LVRKSKPPED VLKGLTPLQG IPEPDLEDAI IRIFSSPNIV SRRWIYEQYD HEVGGRTVVK 

       430        440        450        460        470        480 
PGIGDAAVLK INDDKGIAVK GDGNPRYTWL NPFNGAANVV SECYRNLVSV GSTPLAIVDE 

       490        500        510        520        530        540 
LNAGNPEKPE HYWFFENMLK GLAWISDELG LPVVGGKVSF YNEDSSGNQV KPTLAIVGIG 

       550        560        570        580        590        600 
RVENVRKAKT MEFKEPGSYI VVIGETLPEI GGSEYLYRVH GLEVGEIPVP RPRLEYMHGS 

       610        620        630        640        650        660 
TVRMLINEDA VSSVHDVSIG GLVTSMLEMA VASGLGAEVE LSLVPSRGID RLDYLLFSET 

       670        680        690        700        710        720 
QARYIVEVPL NKVEKARRIL DEMNVVYGVV GRVSSRSQVD LLVNGKRILE LDIGVLREIY 

       730 
ADSLEKYLER G

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References

[1]"Complete genome sequence of the anaerobic, protein-degrading hyperthermophilic crenarchaeon Desulfurococcus kamchatkensis."
Ravin N.V., Mardanov A.V., Beletsky A.V., Kublanov I.V., Kolganova T.V., Lebedinsky A.V., Chernyh N.A., Bonch-Osmolovskaya E.A., Skryabin K.G.
J. Bacteriol. 191:2371-2379(2009) [PubMed: 19114480] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001140 Genomic DNA. Translation: ACL10848.1.
RefSeqYP_002428215.1. NC_011766.1.

3D structure databases

ProteinModelPortalB8D417.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8D417.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7170743.
GenomeReviewsGene locus DKAM_0522 in contig CP001140_GR.
KEGGdka:DKAM_0522.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG311214.
OMAYGNSFGV.
ProtClustDBPRK01213.

Family and domain databases

HAMAPMF_00420. PurL_2.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01736. FGAM_synth_II. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB8D417_DESK1
AccessionPrimary (citable) accession number: B8D417
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info