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B8D2L7 (B8D2L7_DESK1) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase HAMAP-Rule MF_00407 RuleBase RU000617

EC=6.5.1.1 HAMAP-Rule MF_00407 RuleBase RU000617
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig HAMAP-Rule MF_00407
Ordered Locus Names:DKAM_0279 EMBL ACL10605.1
OrganismDesulfurococcus kamchatkensis (strain 1221n / DSM 18924) [Complete proteome] [HAMAP] EMBL ACL10605.1
Taxonomic identifier490899 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeDesulfurococcus

Protein attributes

Sequence length610 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407 RuleBase RU000617

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. HAMAP-Rule MF_00407

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2691N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2671ATP By similarity HAMAP-Rule MF_00407
Binding site2741ATP By similarity HAMAP-Rule MF_00407
Binding site2891ATP By similarity HAMAP-Rule MF_00407
Binding site3191ATP By similarity HAMAP-Rule MF_00407
Binding site3591ATP By similarity HAMAP-Rule MF_00407
Binding site4361ATP By similarity HAMAP-Rule MF_00407
Binding site4421ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
B8D2L7 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: A1B6FF558E889967

FASTA61068,826
        10         20         30         40         50         60 
MSIGQADIPV RLIADVFERI EVITSRIQMT ALLVTLFKQT PPEIIDRVVY LIQGRLWPDW 

        70         80         90        100        110        120 
KELPELGVGE KLLVKAIALA TNSSEAQVED LAKSLGDLGK VAEQLKSRIS GRQQGVTLFS 

       130        140        150        160        170        180 
FTQQQPELTV NKVYDTLVRV AMAQGEGSKD LKIRLLAGLL REASPKEAKF IVRFAEGRLR 

       190        200        210        220        230        240 
VGIGEATVMD ALAITYGGGA AARQVIERAY NLRADLGEVA KILALNGLEA LRNVRPEVGV 

       250        260        270        280        290        300 
PIRPMLAERL SDPKEILEKV GGDAFVEYKY DGERAQIHKK DDKVWIFSRR LENITHQYPD 

       310        320        330        340        350        360 
VIELARDHLK AREVIVEGEI VAYDLDTGEL KPFQELMHRK RKHEVRTALK EYPVKVFLFD 

       370        380        390        400        410        420 
LLYADGRDYT IEPLPVRRGK LLEIVDRTDE FTVAEYIRTS DVKELEKFFL KAISDGAEGV 

       430        440        450        460        470        480 
MVKALHDGSI YQAGTRGWLW IKFKRDYRSE MIDTVDLVVV GAFYGKGRRG GKYGALLMAA 

       490        500        510        520        530        540 
YNDEKDVFET VCKVGSGFKD EDLDKLPEIL KPYIRDRKSP RVVAEMEPDV WVEPALVAEI 

       550        560        570        580        590        600 
IGAELTLSPI HTCAYGRIKS DAGLSIRFPR FIRWRPDKRP EDATTSNELV EMYNQQLKKI 

       610 
AGESKPVEEA 

« Hide

References

[1]"Complete genome sequence of the anaerobic, protein-degrading hyperthermophilic crenarchaeon Desulfurococcus kamchatkensis."
Ravin N.V., Mardanov A.V., Beletsky A.V., Kublanov I.V., Kolganova T.V., Lebedinsky A.V., Chernyh N.A., Bonch-Osmolovskaya E.A., Skryabin K.G.
J. Bacteriol. 191:2371-2379(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1221n / DSM 18924.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001140 Genomic DNA. Translation: ACL10605.1.
RefSeqYP_002427972.1. NC_011766.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING490899.DKAM_0279.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL10605; ACL10605; DKAM_0279.
GeneID7171533.
KEGGdka:DKAM_0279.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAMVKMLEG.

Enzyme and pathway databases

BioCycDKAM490899:GHYH-283-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB8D2L7_DESK1
AccessionPrimary (citable) accession number: B8D2L7
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: June 11, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)