B8D2I3 (DXS_HALOH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Hore_06530

Organism

Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) [Complete proteome] [HAMAP]

Taxonomic identifier

373903 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Halanaerobiales › Halanaerobiaceae › Halothermothrix

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 636	636	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000132936

Sequences

	Sequence		Length	Mass (Da)
	B8D2I3 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 2ACCDA60840BF3C2	FASTA	636	70,037
	10 20 30 40 50 60 MGNIFDKVNS PEDLKSLNIE DLNSLAQEVR KFIIETVADT GGHLASNLGV VELTLALHYH 70 80 90 100 110 120 LNSPKDKIIW DVGHQSYTHK ILTGRKKKFH TIRQYKGLSG FPKYSESIHD VIETGHSSTS 130 140 150 160 170 180 ISAALGLALA RDLKNRNDRI YAVIGDGALT GGMAFEALNH AGHLGTDIKV VLNDNAMSIS 190 200 210 220 230 240 KNVGALSHYL SRIRTDPTLS KFKDDVEVLL SRIPKIGNTV SRSVERVKDG LKYLFLSGVL 250 260 270 280 290 300 FEEMGFTYMG PLDGHNIQEL ITNFKNADNI KGPVLIHVNT KKGKGYKPAE SQPSKFHGVS 310 320 330 340 350 360 PFKIDNGESK RKKSNFTYSQ VFGQTMVKLG NKDKKIVGIT AAMPEGTGLS YFKKEFPDRF 370 380 390 400 410 420 FDVGIAEQHA VTLATGMARA GMKPVVAIYS TFLQRAYDQV IHDACIQNLP VTFAIDRAGI 430 440 450 460 470 480 VGADGETHHG LFDLSFLRAI PNIIIMAPKN ENELQHMIYT AINNDQPVAI RYPRGEGYGV 490 500 510 520 530 540 ELDNDFSTIP IGKGELLCDG KDVLIIAVGS RVYPAMEAAR VLSQQGIKAA VINARFIKPL 550 560 570 580 590 600 DKNLILNKIN ECKKVITVEE HALKGGFGSA ILEFINENDL RGIKVKRLGL PDRFLPHGPT 610 620 630 GHLQTIYHID KNAIIESALK LVDERVELGL WPGKNA

References

[1]

"Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii." Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C. PLoS ONE 4:E4192-E4192(2009) [PubMed: 19145256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H 168 / OCM 544 / DSM 9562.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001098 Genomic DNA. Translation: ACL69410.1.
RefSeq	YP_002508405.1. NC_011899.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8D2I3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7314559.
GenomeReviews	Gene locus Hore_06530 in contig CP001098_GR.
KEGG	hor:Hore_06530.
PATRIC	22100652. VBIHalOre56292_0701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	RAHEFES.
ProtClustDB	CLSK2806258.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_HALOH

Accession

Primary (citable) accession number: B8D2I3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program