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B8D1K5

- PFKA_HALOH

UniProt

B8D1K5 - PFKA_HALOH

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, Hore_03210
Organism
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogen By similarity
Metal bindingi103 – 1031Magnesium; catalytic By similarity
Active sitei128 – 1281Proton acceptor By similarity
Binding sitei155 – 1551Allosteric activator ADP By similarity
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarity
Binding sitei212 – 2121Allosteric activator ADP By similarity
Binding sitei223 – 2231Substrate By similarity
Binding sitei256 – 2561Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarity
Nucleotide bindingi102 – 1054ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHORE373903:GHB1-344-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:Hore_03210
OrganismiHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Taxonomic identifieri373903 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalothermothrix
ProteomesiUP000000719: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_1000192374Add
BLAST

Proteomic databases

PRIDEiB8D1K5.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi373903.Hore_03210.

Structurei

3D structure databases

ProteinModelPortaliB8D1K5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni126 – 1283Substrate binding By similarity
Regioni170 – 1723Substrate binding By similarity
Regioni186 – 1883Allosteric activator ADP binding By similarity
Regioni214 – 2163Allosteric activator ADP binding By similarity
Regioni262 – 2654Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiAKLERGH.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8D1K5-1 [UniParc]FASTAAdd to Basket

« Hide

MKKIGVLTSG GDAPGMNAAI RSVVRTGIYH GLDVVGIRRG YAGLIEGDFY    50
LMDRGSVADI IQRGGTILLS ARCEEFKTEE GRQKALKKMK EEGIEGLVVI 100
GGDGSFRGAE KVSKELGIPA IGIPGTIDND LACTDFTIGF DTAMNTIIDT 150
INKIRDTATS HERTFVVETM GRRSGFLTLM AGLAGGAESI LIPEIDFDLD 200
DVSQKLKKGY ERGKLHSIIL VAEGVGDDFS TNRDINHSKA FVIGKQIAEK 250
TGLETRVIIL GHIQRGGSPT AMDRILASRM GSKAVELLLA SESNKMVGYV 300
NNKLVTCDIS EALSKEKQVE KDVYKLANIL SM 332
Length:332
Mass (Da):35,770
Last modified:March 3, 2009 - v1
Checksum:i12B78E782FFC46BF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001098 Genomic DNA. Translation: ACL69082.1.
RefSeqiYP_002508077.1. NC_011899.1.

Genome annotation databases

EnsemblBacteriaiACL69082; ACL69082; Hore_03210.
GeneIDi7314707.
KEGGihor:Hore_03210.
PATRICi22099908. VBIHalOre56292_0341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001098 Genomic DNA. Translation: ACL69082.1 .
RefSeqi YP_002508077.1. NC_011899.1.

3D structure databases

ProteinModelPortali B8D1K5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 373903.Hore_03210.

Proteomic databases

PRIDEi B8D1K5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL69082 ; ACL69082 ; Hore_03210 .
GeneIDi 7314707.
KEGGi hor:Hore_03210.
PATRICi 22099908. VBIHalOre56292_0341.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248870.
KOi K00850.
OMAi AKLERGH.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci HORE373903:GHB1-344-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii."
    Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.
    PLoS ONE 4:E4192-E4192(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H 168 / OCM 544 / DSM 9562.

Entry informationi

Entry nameiPFKA_HALOH
AccessioniPrimary (citable) accession number: B8D1K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: July 9, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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