B8D1G8 (ARGB_HALOH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	Hore_02840

Organism

Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) [Complete proteome] [HAMAP]

Taxonomic identifier

373903 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Halanaerobiales › Halanaerobiaceae › Halothermothrix

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Acetylglutamate kinase HAMAP MF_00082_B		PRO_1000118352
Regions
	Region	64 – 65	2	Substrate binding By similarity
Sites
	Binding site	86	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Site	29	1	Transition state stabilizer By similarity
	Site	246	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8D1G8 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: B244D393345CF787	FASTA	290	31,411
	10 20 30 40 50 60 MEELINKAQT LVEALPYIRN FYGKTFVIKY GGSTMGDKRL KEKIMEDITL LKYVGVNPVL 70 80 90 100 110 120 IHGGGPAISE AMESMDKEAR FIQGLRITDS ETMSLVEMVL VGKVNKELVS IINRLGGEAV 130 140 150 160 170 180 GICGKDGGLI EADKLIFEDK SIDLGHVGSV KKINPEIVLN LIKGGYIPVI SPVGSSKEGD 190 200 210 220 230 240 TYNINADTVA GKLAVSLKAE KLIFLTDVDG VWSDPSNEKT RVSSLTVNEV KSWIGEGKIK 250 260 270 280 290 GGMVPKVEAC IEAVTCGVNR THILNGLIPH ALLLEIFTDR GIGTMILKER

References

[1]

"Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii." Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C. PLoS ONE 4:E4192-E4192(2009) [PubMed: 19145256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H 168 / OCM 544 / DSM 9562.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001098 Genomic DNA. Translation: ACL69045.1.
RefSeq	YP_002508040.1. NC_011899.1.
3D structure databases
ProteinModelPortal	B8D1G8.
SMR	B8D1G8. Positions 6-289.
ModBase	Search...
Protein-protein interaction databases
STRING	B8D1G8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7314670.
GenomeReviews	Gene locus Hore_02840 in contig CP001098_GR.
KEGG	hor:Hore_02840.
PATRIC	22099830. VBIHalOre56292_0302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497643.
OMA	GGNAMID.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_HALOH

Accession

Primary (citable) accession number: B8D1G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program