B8D0W1 (ALR_HALOH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	Hore_01680

Organism

Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) [Complete proteome] [HAMAP]

Taxonomic identifier

373903 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Halanaerobiales › Halanaerobiaceae › Halothermothrix

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Alanine racemase HAMAP MF_01201		PRO_1000164600
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	267	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8D0W1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 4B8C82F948689246	FASTA	379	42,138
	10 20 30 40 50 60 MNKRITRPTW AEIDLSCLQF NFNQVKEILG SNVKIMSVVK ADAYGHGVIP VAKTLVEAGT 70 80 90 100 110 120 QRLAVAIPEE GVELREAGLS VPIQVLGEVL PSQYELLFKY DLIPTVGREE TALSLNRLAA 130 140 150 160 170 180 KYNVVKKVHI KVDTGMGRIG VRPREAVGFV KKVNSLSNIK IEGLMTHFAS ADERDKSYTY 190 200 210 220 230 240 EQWDKFKQVL DGLNKLRIDI PIKHSSNSAA IIDFKKFGLD MVRPGIMLYG LKPSRDLINN 250 260 270 280 290 300 IDLKPVLTWK TRIVYLKEVP PGTGISYGTT YVTNRKSKIA TLPVGYADGY PRILSNQGQV 310 320 330 340 350 360 LVRGRKAPIR GRVCMDQIMI DVTEIPDVRV GDEVVLIGEQ GTQKISATDI AEKADTINYE 370 IVCGISQRVP REYKNKIGG

References

[1]

"Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii." Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C. PLoS ONE 4:E4192-E4192(2009) [PubMed: 19145256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H 168 / OCM 544 / DSM 9562.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001098 Genomic DNA. Translation: ACL68930.1.
RefSeq	YP_002507925.1. NC_011899.1.
3D structure databases
ProteinModelPortal	B8D0W1.
ModBase	Search...
Protein-protein interaction databases
STRING	B8D0W1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7313296.
GenomeReviews	Gene locus Hore_01680 in contig CP001098_GR.
KEGG	hor:Hore_01680.
PATRIC	22099586. VBIHalOre56292_0181.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG712172.
OMA	SVELWGD.
ProtClustDB	CLSK2806173.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_HALOH

Accession

Primary (citable) accession number: B8D0W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program