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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 708ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHORE373903:GHB1-77-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:Hore_00600
OrganismiHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Taxonomic identifieri373903 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalothermothrix
ProteomesiUP000000719 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Formate--tetrahydrofolate ligasePRO_1000185255Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi373903.Hore_00600.

Structurei

3D structure databases

ProteinModelPortaliB8D067.
SMRiB8D067. Positions 4-552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8D067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSDIEIAQD ARMEPIEEIA EKAGLHKDQI ELYGKYKAKV NTDIIDDDKK
60 70 80 90 100
DGKLVLVTAI TPTPAGEGKT TTTVGLGQAL NLLGKKAIIA LREPSLGPTM
110 120 130 140 150
GIKGGAAGGG YSQVLPMEDI NLHFTGDIHA IGAAHNLLSA VIDNHIKQGN
160 170 180 190 200
DLGIDPTSVT WKRVVDMNDR ALRNIVVGLG GKAHGVPRED GFMITVASEI
210 220 230 240 250
MAILCLANNL EDLKNKISNI VVGYTYDGEA ITAGDLKVSG AMTALLKDAI
260 270 280 290 300
KPNLVQTLEN TPAFIHGGPF ANIAHGCNSI MATKMALKLG EITVTEAGFG
310 320 330 340 350
ADLGAEKFFN IKCRYAGLNP DAAVVVATVR ALKMHGGVDK DSLSEENLDA
360 370 380 390 400
LARGFENLEK HLENVSKFGV PSVVAINRFP GDTEAELNLV RDKCEEMGVP
410 420 430 440 450
VAISEVWARG GEGGLDLARK LVDVLENTPG QFSYLYDVNF PITDKIEKIA
460 470 480 490 500
KEIYGADGVN YTSKALKQID KYTELGYDKL PICMAKTQSS ISDDPSLKGR
510 520 530 540 550
PRGFRINVRE VNLSAGAGFI IPLTGPVLTM PGLPKKPAAE GIDIDADGKI

SGLF
Length:554
Mass (Da):59,069
Last modified:March 3, 2009 - v1
Checksum:i2771AA32B81C6321
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001098 Genomic DNA. Translation: ACL68821.1.
RefSeqiWP_012635020.1. NC_011899.1.
YP_002507816.1. NC_011899.1.

Genome annotation databases

EnsemblBacteriaiACL68821; ACL68821; Hore_00600.
KEGGihor:Hore_00600.
PATRICi22099348. VBIHalOre56292_0066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001098 Genomic DNA. Translation: ACL68821.1.
RefSeqiWP_012635020.1. NC_011899.1.
YP_002507816.1. NC_011899.1.

3D structure databases

ProteinModelPortaliB8D067.
SMRiB8D067. Positions 4-552.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi373903.Hore_00600.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL68821; ACL68821; Hore_00600.
KEGGihor:Hore_00600.
PATRICi22099348. VBIHalOre56292_0066.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciHORE373903:GHB1-77-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii."
    Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.
    PLoS ONE 4:E4192-E4192(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H 168 / OCM 544 / DSM 9562.

Entry informationi

Entry nameiFTHS_HALOH
AccessioniPrimary (citable) accession number: B8D067
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 29, 2015
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.