B8CZC3 (LPXK_HALOH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	Hore_18940

Organism

Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) [Complete proteome] [HAMAP]

Taxonomic identifier

373903 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Halanaerobiales › Halanaerobiaceae › Halothermothrix

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 384	384	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000134742
Regions
	Nucleotide binding	72 – 79	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B8CZC3 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: E412EB3D196816CC	FASTA	384	43,216
	10 20 30 40 50 60 MVANMRKKLE SYLINLIKSG PRSICDKIVL FILSILEKIY NLLILLRRLL YNLNLVKPGE 70 80 90 100 110 120 VEARVISVGN ITAGGTGKTP LVIYLAKKLA EENRVVVISR GYQSQSEGEE PSVVSDGRNI 130 140 150 160 170 180 LTDVSEAGDE VYMMATLLGG VPLITGSNRY KAARLASRRF NAEIIILDDG FQHWQLKRDV 190 200 210 220 230 240 DIVMIDGLKP FGQGRLIPRG FLREPLSGLK RADFFVISRA HHISREKLQE IKDTLCQYNQ 250 260 270 280 290 300 NAVVYEATTS SVYLKELSVA SLEMKSIIHK KRPLDELKGA KVIAVCGLGN PRSFYRDLEI 310 320 330 340 350 360 SGAEVIETLS FNDHHQYRPD DFDKIINLAR QKAIDIVITT EKDAVKFSRD DIKKFIDHNI 370 380 NLYVLGIEIS LKGTVDLAQV INKY

References

[1]

"Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii." Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C. PLoS ONE 4:E4192-E4192(2009) [PubMed: 19145256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H 168 / OCM 544 / DSM 9562.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001098 Genomic DNA. Translation: ACL70642.1.
RefSeq	YP_002509637.1. NC_011899.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8CZC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7312709.
GenomeReviews	Gene locus Hore_18940 in contig CP001098_GR.
KEGG	hor:Hore_18940.
PATRIC	22103338. VBIHalOre56292_2019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	CLSK2806570.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_HALOH

Accession

Primary (citable) accession number: B8CZC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program