B8CY74 (CLPP_HALOH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp

Gene names

Name:	clpP
Ordered Locus Names:	Hore_14940

Organism

Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) [Complete proteome] [HAMAP]

Taxonomic identifier

373903 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Halanaerobiales › Halanaerobiaceae › Halothermothrix

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	ATP-dependent Clp protease proteolytic subunit HAMAP MF_00444		PRO_1000206155
Sites
	Active site	98	1	By similarity
	Active site	123	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8CY74 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: FCACE97A0867006F	FASTA	198	21,980
	10 20 30 40 50 60 MSLIPVVVEQ TNRGERSYDI YSRLLKDRIV FLGHPINDEL SNLIIAQLLF LEAEDPDKDI 70 80 90 100 110 120 HLYINSPGGS VTAALAMYDT IQYIKPDVAT ICMGQAASAG ALLLASGTKG KRYALPNARV 130 140 150 160 170 180 MIHQPAGGVQ GKATEAEIHI KELLRLRERL NEILSKHTGK SVEQISKDVE QDYFMTAEEA 190 LEYGIIDEVI TKNELKDK

References

[1]

"Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii." Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C. PLoS ONE 4:E4192-E4192(2009) [PubMed: 19145256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H 168 / OCM 544 / DSM 9562.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001098 Genomic DNA. Translation: ACL70243.1.
RefSeq	YP_002509238.1. NC_011899.1.
3D structure databases
ProteinModelPortal	B8CY74.
SMR	B8CY74. Positions 2-191.
ModBase	Search...
Protein-protein interaction databases
STRING	B8CY74.
Protein family/group databases
MEROPS	S14.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7313085.
GenomeReviews	Gene locus Hore_14940 in contig CP001098_GR.
KEGG	hor:Hore_14940.
PATRIC	22102462. VBIHalOre56292_1588.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	CTICIGL.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. ClpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP_HALOH

Accession

Primary (citable) accession number: B8CY74

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program