Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei588 – 5881ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHORE373903:GHB1-1389-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Hore_13370
OrganismiHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Taxonomic identifieri373903 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalothermothrix
ProteomesiUP000000719 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830Leucine--tRNA ligasePRO_1000199209Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi373903.Hore_13370.

Structurei

3D structure databases

ProteinModelPortaliB8CXR8.
SMRiB8CXR8. Positions 1-766.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi585 – 5895"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8CXR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGYYNFADI EKKWQDKWEK DGLYKTQEET DKDNYYVLEM FPYPSGNLHM
60 70 80 90 100
GHVRVYSIGD VIARFKRMNG YNVLHPMGWD AFGLPAENAA IKHGNIHPQD
110 120 130 140 150
WTWDNIKNMK KQMKSLGLSY DWDREVTTAK EDYYKWTQWF FVKMFKKGLA
160 170 180 190 200
YKKKAAVNWC PGCETVLANE QVVNNACERC GTEVEEKELE QWFFKITNYA
210 220 230 240 250
ERLLEDHKLL QNWPEKVKIM QRNWIGRSEG MRIKFPVKGS SEEIEVFTTR
260 270 280 290 300
PDTIFGATYM VLAPEHPLVE KLISGTEKEK EVRQFIDRVK KQKEMERTSP
310 320 330 340 350
ESEKEGLFTG AYAINPMTGE EIPIMIANYV LMGYGTGAIM AVPAHDQRDF
360 370 380 390 400
DFARKYDLPI RVVIQPEERE EELKDTDLNE AYEGSGHLIN SDKYNGLTVK
410 420 430 440 450
EAFDVMAEDM EKEGIGKREV NYRLRDWLIS RQRYWGTPIP IVYCEKCGTV
460 470 480 490 500
PVPEEELPVV LPRDVEFSPT GESPLAKVDE FVNTTCPVCG GKARRETDTM
510 520 530 540 550
DTFVDSSWYF LRYTDPKNDK LPFSKENAKK WFPVDQYIGG IEHAILHLLY
560 570 580 590 600
ARFFTKVIYD MDMIDSVEPF TNWLAQGMVL KDGAKMSKSK GNVVDPEDIL
610 620 630 640 650
DRFGADTARL FILFAAPPEK DLEWSDRGVE GAERFLNRVW RLVADNIKEI
660 670 680 690 700
KNTDQASLDV NSFNKNEKDL YRNLHVTIKR VTEDIGERLN FNTAISAIME
710 720 730 740 750
LTNATYQYLN GVEKVNYTLI KDIIEKMLLI LAPFAPHMTE ELWSELGNDE
760 770 780 790 800
SIHIQKWPGY EEKALKKDEV TIVVQVNGKV RDKLQVSADI DEDKLKEQVL
810 820 830
ELPRIQKYTE GKEIVKTIII PKKLVNIVVK
Length:830
Mass (Da):96,269
Last modified:March 3, 2009 - v1
Checksum:i9BB05557D27E2CE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001098 Genomic DNA. Translation: ACL70087.1.
RefSeqiWP_012636271.1. NC_011899.1.
YP_002509082.1. NC_011899.1.

Genome annotation databases

EnsemblBacteriaiACL70087; ACL70087; Hore_13370.
KEGGihor:Hore_13370.
PATRICi22102120. VBIHalOre56292_1419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001098 Genomic DNA. Translation: ACL70087.1.
RefSeqiWP_012636271.1. NC_011899.1.
YP_002509082.1. NC_011899.1.

3D structure databases

ProteinModelPortaliB8CXR8.
SMRiB8CXR8. Positions 1-766.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi373903.Hore_13370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL70087; ACL70087; Hore_13370.
KEGGihor:Hore_13370.
PATRICi22102120. VBIHalOre56292_1419.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciHORE373903:GHB1-1389-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii."
    Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.
    PLoS ONE 4:E4192-E4192(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H 168 / OCM 544 / DSM 9562.

Entry informationi

Entry nameiSYL_HALOH
AccessioniPrimary (citable) accession number: B8CXR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 1, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.