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B8CWL4

- SYI_HALOH

UniProt

B8CWL4 - SYI_HALOH

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei560 – 5601Aminoacyl-adenylateUniRule annotation
Binding sitei604 – 6041ATPUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi901 – 9011ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHORE373903:GHB1-972-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Hore_09270
OrganismiHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Taxonomic identifieri373903 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalothermothrix
ProteomesiUP000000719: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Isoleucine--tRNA ligasePRO_1000189169Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi373903.Hore_09270.

Structurei

3D structure databases

ProteinModelPortaliB8CWL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi601 – 6055"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8CWL4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKETINLP RTEFPMRANL SKREPGIEKY WEENNVYEKA IKNREGNKQF
60 70 80 90 100
ILHDGPPYAN GDIHIGHALN KILKDIVTRY KTLSGYYSPY VPGWDTHGLP
110 120 130 140 150
IEHKVTKEMG DKAKDLSVSE LRDKCRDYAL KYVERQKEQF KRLGVWGEWD
160 170 180 190 200
KPYLTLNPEY EVKQIEVFGE MAKRGLFYKG KKPVHWCPSC ETALAEAEVE
210 220 230 240 250
YGEHRSPSIY VKFPLKDKVN VGGVELTPGD SYVVIWTTTP WTIPANMAIA
260 270 280 290 300
LHPEFDYVVV KASGEKLVMA RELVDRVMEE AEVENYKVVG QAFKGTELEN
310 320 330 340 350
KRCRHPFEDR DSILILGDHV TLEQGTGCVH TAPGHGHDDY LVGLKYDLDI
360 370 380 390 400
YAPMDNRGVF TEEAERFAGM YYDDVNKEVT KILDEKGLLM NLSFISHQYP
410 420 430 440 450
HCWRCKGALI FRATDQWFAS IEAIKEEALK AIHSVDWYPA WGEERMANMI
460 470 480 490 500
SERTDWCISR QKKWGVPIPV FYCKDCGHSI INDETIEAVK KLFAREGSSG
510 520 530 540 550
WYKYSAEEIL PEGFSCPECG GKSFKKEEDI MDVWFDSGSS HKAVLETRDH
560 570 580 590 600
LSWPSQLYLE GTDQYRGWFN SSLLTAVATE GKPPYEAVVT NGFVVDDKGN
610 620 630 640 650
KMSKSIGNVV SPQDVIKRYG ADILRLWVAS SNFKDDVRVS DRILKQNSEV
660 670 680 690 700
YRRIRNTFRF ILGNINDFNP GQHYVDYNDR AEIDRWIMIK LQDLIKDVTR
710 720 730 740 750
AYDEYEYHRV YHDVHNFCTI EMSSLYVDIV KDRLYTDGTN SLSRRSAQST
760 770 780 790 800
LYDILLVLVK LVAPVLAHTA EEVWQHLDES SKDAESIFLT DWPEVVNEYY
810 820 830 840 850
DDRLMSKWDK LLEVRKDVAK ALELSRENKE IGNSLDARVI LVPVDDKQRQ
860 870 880 890 900
LLKDNIDILP DLFIVSQVEL QGETGEGFHK GSETGISVSV VKASGEKCAR
910 920
CWKYSEAVGN DEEHPELCER CVEVVRTEV
Length:929
Mass (Da):106,890
Last modified:March 3, 2009 - v1
Checksum:iCADD37AE9394EAFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001098 Genomic DNA. Translation: ACL69683.1.
RefSeqiYP_002508678.1. NC_011899.1.

Genome annotation databases

EnsemblBacteriaiACL69683; ACL69683; Hore_09270.
GeneIDi7313420.
KEGGihor:Hore_09270.
PATRICi22101240. VBIHalOre56292_0986.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001098 Genomic DNA. Translation: ACL69683.1 .
RefSeqi YP_002508678.1. NC_011899.1.

3D structure databases

ProteinModelPortali B8CWL4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 373903.Hore_09270.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL69683 ; ACL69683 ; Hore_09270 .
GeneIDi 7313420.
KEGGi hor:Hore_09270.
PATRICi 22101240. VBIHalOre56292_0986.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HORE373903:GHB1-972-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii."
    Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.
    PLoS ONE 4:E4192-E4192(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H 168 / OCM 544 / DSM 9562.

Entry informationi

Entry nameiSYI_HALOH
AccessioniPrimary (citable) accession number: B8CWL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3