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B8CWL4

- SYI_HALOH

UniProt

B8CWL4 - SYI_HALOH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei560 – 5601Aminoacyl-adenylateUniRule annotation
    Binding sitei604 – 6041ATPUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHORE373903:GHB1-972-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Hore_09270
    OrganismiHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
    Taxonomic identifieri373903 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalothermothrix
    ProteomesiUP000000719: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 929929Isoleucine--tRNA ligasePRO_1000189169Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi373903.Hore_09270.

    Structurei

    3D structure databases

    ProteinModelPortaliB8CWL4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi601 – 6055"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8CWL4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKETINLP RTEFPMRANL SKREPGIEKY WEENNVYEKA IKNREGNKQF    50
    ILHDGPPYAN GDIHIGHALN KILKDIVTRY KTLSGYYSPY VPGWDTHGLP 100
    IEHKVTKEMG DKAKDLSVSE LRDKCRDYAL KYVERQKEQF KRLGVWGEWD 150
    KPYLTLNPEY EVKQIEVFGE MAKRGLFYKG KKPVHWCPSC ETALAEAEVE 200
    YGEHRSPSIY VKFPLKDKVN VGGVELTPGD SYVVIWTTTP WTIPANMAIA 250
    LHPEFDYVVV KASGEKLVMA RELVDRVMEE AEVENYKVVG QAFKGTELEN 300
    KRCRHPFEDR DSILILGDHV TLEQGTGCVH TAPGHGHDDY LVGLKYDLDI 350
    YAPMDNRGVF TEEAERFAGM YYDDVNKEVT KILDEKGLLM NLSFISHQYP 400
    HCWRCKGALI FRATDQWFAS IEAIKEEALK AIHSVDWYPA WGEERMANMI 450
    SERTDWCISR QKKWGVPIPV FYCKDCGHSI INDETIEAVK KLFAREGSSG 500
    WYKYSAEEIL PEGFSCPECG GKSFKKEEDI MDVWFDSGSS HKAVLETRDH 550
    LSWPSQLYLE GTDQYRGWFN SSLLTAVATE GKPPYEAVVT NGFVVDDKGN 600
    KMSKSIGNVV SPQDVIKRYG ADILRLWVAS SNFKDDVRVS DRILKQNSEV 650
    YRRIRNTFRF ILGNINDFNP GQHYVDYNDR AEIDRWIMIK LQDLIKDVTR 700
    AYDEYEYHRV YHDVHNFCTI EMSSLYVDIV KDRLYTDGTN SLSRRSAQST 750
    LYDILLVLVK LVAPVLAHTA EEVWQHLDES SKDAESIFLT DWPEVVNEYY 800
    DDRLMSKWDK LLEVRKDVAK ALELSRENKE IGNSLDARVI LVPVDDKQRQ 850
    LLKDNIDILP DLFIVSQVEL QGETGEGFHK GSETGISVSV VKASGEKCAR 900
    CWKYSEAVGN DEEHPELCER CVEVVRTEV 929
    Length:929
    Mass (Da):106,890
    Last modified:March 3, 2009 - v1
    Checksum:iCADD37AE9394EAFF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001098 Genomic DNA. Translation: ACL69683.1.
    RefSeqiYP_002508678.1. NC_011899.1.

    Genome annotation databases

    EnsemblBacteriaiACL69683; ACL69683; Hore_09270.
    GeneIDi7313420.
    KEGGihor:Hore_09270.
    PATRICi22101240. VBIHalOre56292_0986.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001098 Genomic DNA. Translation: ACL69683.1 .
    RefSeqi YP_002508678.1. NC_011899.1.

    3D structure databases

    ProteinModelPortali B8CWL4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 373903.Hore_09270.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL69683 ; ACL69683 ; Hore_09270 .
    GeneIDi 7313420.
    KEGGi hor:Hore_09270.
    PATRICi 22101240. VBIHalOre56292_0986.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci HORE373903:GHB1-972-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii."
      Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.
      PLoS ONE 4:E4192-E4192(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H 168 / OCM 544 / DSM 9562.

    Entry informationi

    Entry nameiSYI_HALOH
    AccessioniPrimary (citable) accession number: B8CWL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3