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B8CWI5 (DDL_HALOH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Hore_08980
OrganismHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) [Complete proteome] [HAMAP]
Taxonomic identifier373903 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalothermothrix

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 343343D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000189738

Regions

Domain132 – 337206ATP-grasp
Nucleotide binding165 – 22056ATP By similarity

Sites

Metal binding2911Magnesium or manganese 1 By similarity
Metal binding3041Magnesium or manganese 1 By similarity
Metal binding3041Magnesium or manganese 2 By similarity
Metal binding3061Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B8CWI5 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: D3EBCF53ACBBBCD3

FASTA34338,656
        10         20         30         40         50         60 
MSKLRLGVLF GGRSQEHEVS VMSARSVVKM AKKEKYKVIP FGITKKGQWV GPEESWNILN 

        70         80         90        100        110        120 
NGINEVKASK DRCITESVRV FLDHKLDIVF PVLHGPYGED GKLQGFLDCL DIPYIGASVL 

       130        140        150        160        170        180 
SSAAGMDKEI MKNLFSYHKI PQARYRVYRQ NHLENGLDGI ISEIDRFLGW PCFVKPANMG 

       190        200        210        220        230        240 
SSIGVSKVHS PGEVKKALEK GFYYDRKLIF EEFVEGREIE CSVLGNDQVE ASLPGEILPG 

       250        260        270        280        290        300 
KEFYDYEAKY KDNKTRLVIP ASLDESVIDK ARNLAIKAFK AIDGNGFARV DFFLKNNGVL 

       310        320        330        340 
LVNEINTIPG FTQYSMYPKL WEATGLNYPD LIDKLIELAL EDR 

« Hide

References

[1]"Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii."
Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.
PLoS ONE 4:E4192-E4192(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H 168 / OCM 544 / DSM 9562.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001098 Genomic DNA. Translation: ACL69654.1.
RefSeqYP_002508649.1. NC_011899.1.

3D structure databases

ProteinModelPortalB8CWI5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING373903.Hore_08980.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL69654; ACL69654; Hore_08980.
GeneID7314894.
KEGGhor:Hore_08980.
PATRIC22101176. VBIHalOre56292_0954.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycHORE373903:GHB1-943-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_HALOH
AccessionPrimary (citable) accession number: B8CWI5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways