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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi492MagnesiumUniRule annotation1
Metal bindingi498MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciHORE373903:GHB1-826-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Hore_07900
OrganismiHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Taxonomic identifieri373903 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalothermothrix
Proteomesi
  • UP000000719 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001857391 – 705Polyribonucleotide nucleotidyltransferaseAdd BLAST705

Interactioni

Protein-protein interaction databases

STRINGi373903.Hore_07900.

Structurei

3D structure databases

ProteinModelPortaliB8CW78.
SMRiB8CW78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini559 – 618KHUniRule annotationAdd BLAST60
Domaini628 – 696S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRYMHNYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

B8CW78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKDWTIDVA GGKMKFETGK YAKQANGSVV ARYGDTTVLV TATMSEPREG
60 70 80 90 100
IDYFPLMVNY EERVYAIGKI PGSITRREGR PRDVATLAAR LIDRPLRPLF
110 120 130 140 150
PEGFRHDVQI IATVLSVDND CEPDILALNG ASVALTLSDI PFDGPIGGVK
160 170 180 190 200
VGLVDGELVI NPDEEEREKS KLDLTVAGTR DAVLMVEAGA NEVSEDVMLD
210 220 230 240 250
AIELAHQEIK RLVLLQEEIG EEAGKKKFEF TKDEITPELD EEIRKYISSD
260 270 280 290 300
MENALRIPEK LERNAKVDEI KENTLQYFED MFENNGLDNE EKNKQLKMVE
310 320 330 340 350
RTIEKVMKEK VRKMIIEEGI RPDGRKPDEI RPIWCEVGTL PRVHGSGVFT
360 370 380 390 400
RGQTQALSVV TLGATSDEQI LFGLGEEETK RYMHHYNFPP YSVGETSPLR
410 420 430 440 450
SPGRREIGHG ALGERALQPV IPDQEEFPYT IRVVSEVLES NGSTSQASIC
460 470 480 490 500
GSTLALMDAG VPIKEPVAGI AMGLLKEDEK VVILSDIQGL EDFYGDMDFK
510 520 530 540 550
VAGTRNGITA LQMDIKIHGI SKEILKKALK RAREGRLYIL DKMLQVIDKP
560 570 580 590 600
RPELSPYAPL MITMKVSPDK IRHIIGPGGK IINKIIDETG VEIDIDDDGS
610 620 630 640 650
VYILAQDQES GNRAKEIINK LTKEVEVGDI YEGRVKKITN FGAFVEILPG
660 670 680 690 700
REGLVHISEL ADHHVKKVED IVKIGDRIPV KVIEIDELGR INLSRKRALK

EQKKE
Length:705
Mass (Da):78,672
Last modified:March 3, 2009 - v1
Checksum:i481278DC741A53AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001098 Genomic DNA. Translation: ACL69547.1.
RefSeqiWP_012635735.1. NC_011899.1.

Genome annotation databases

EnsemblBacteriaiACL69547; ACL69547; Hore_07900.
KEGGihor:Hore_07900.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPNP_HALOH
AccessioniPrimary (citable) accession number: B8CW78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: June 7, 2017
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families