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B8CVY1 (GLGB_HALOH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Hore_06930
OrganismHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) [Complete proteome] [HAMAP]
Taxonomic identifier373903 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalothermothrix

Protein attributes

Sequence length630 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6306301,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000147759

Sites

Active site3081Nucleophile By similarity
Active site3611Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
B8CVY1 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: C07E9BEBE09C7775

FASTA63074,401
        10         20         30         40         50         60 
MKDKIPTDYD RYLFHQGSHY KSYEFLGAHP CEENGKTGVR FSVWAPNAAE VRVIGSFNNW 

        70         80         90        100        110        120 
DGNINPMERI NDSGIWTTFI PEAKKGDLYK YEILTKYGDT RIKSDPYAFY AERKPKTASI 

       130        140        150        160        170        180 
VYSLNNYKWN DKKWMDKKKD YTVYNKPVLI YEVHLGSWKR KENGDYLTYR ELANELVEYV 

       190        200        210        220        230        240 
KDMGYNYIEL LPVAEHPFDG SWGYQLTNYY SVTSRYGTPE DFMYFIDKCH QNGIGVILDW 

       250        260        270        280        290        300 
VPGHFCKDDH GLRLFDGTAL YEPQDPRKAE NEWDTLNFDF NQPEVWSFLI SNAVFWFDIY 

       310        320        330        340        350        360 
HIDGLRVDAV SNLLYLNHGK GDGEWVPNKY GGHENLEAID FIKTLNKVIF EYFPNPLMIA 

       370        380        390        400        410        420 
EESSAWPLVT YPAHLGGLGF NYKWNMGWMN DTLEYMEMDS IYRKYHHNLL TFSFMYMFSE 

       430        440        450        460        470        480 
NFVLPLSHDE VVHGKKSLLD KMPGDYWQKF ANLRTLYGYM MGHPGKKLLF MGGEFGQFIE 

       490        500        510        520        530        540 
WNYKQGLDWL LLDYDMHKKL QNYVRELNYF YRQEKTLWEG DHEDGGFEWI DPHDSEQSII 

       550        560        570        580        590        600 
TFMRKNKDGS DYTIIVCNFT PEVRHNYRIG VPEFKEYKEV FNSDLEKFGG SGQKNSTIIQ 

       610        620        630 
PSEQPWHNRP YSIEITIPPL ATIFFKPLNQ 

« Hide

References

[1]"Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii."
Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.
PLoS ONE 4:E4192-E4192(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H 168 / OCM 544 / DSM 9562.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001098 Genomic DNA. Translation: ACL69450.1.
RefSeqYP_002508445.1. NC_011899.1.

3D structure databases

ProteinModelPortalB8CVY1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING373903.Hore_06930.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL69450; ACL69450; Hore_06930.
GeneID7312928.
KEGGhor:Hore_06930.
PATRIC22100738. VBIHalOre56292_0744.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAECHQNNI.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycHORE373903:GHB1-729-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_HALOH
AccessionPrimary (citable) accession number: B8CVY1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries