Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei308 – 3081NucleophileUniRule annotation
Active sitei361 – 3611Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciHORE373903:GHB1-729-MONOMER.
UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Hore_06930
OrganismiHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Taxonomic identifieri373903 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaHalanaerobialesHalanaerobiaceaeHalothermothrix
ProteomesiUP000000719: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6306301,4-alpha-glucan branching enzyme GlgBPRO_1000147759Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi373903.Hore_06930.

Structurei

3D structure databases

ProteinModelPortaliB8CVY1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

B8CVY1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDKIPTDYD RYLFHQGSHY KSYEFLGAHP CEENGKTGVR FSVWAPNAAE
60 70 80 90 100
VRVIGSFNNW DGNINPMERI NDSGIWTTFI PEAKKGDLYK YEILTKYGDT
110 120 130 140 150
RIKSDPYAFY AERKPKTASI VYSLNNYKWN DKKWMDKKKD YTVYNKPVLI
160 170 180 190 200
YEVHLGSWKR KENGDYLTYR ELANELVEYV KDMGYNYIEL LPVAEHPFDG
210 220 230 240 250
SWGYQLTNYY SVTSRYGTPE DFMYFIDKCH QNGIGVILDW VPGHFCKDDH
260 270 280 290 300
GLRLFDGTAL YEPQDPRKAE NEWDTLNFDF NQPEVWSFLI SNAVFWFDIY
310 320 330 340 350
HIDGLRVDAV SNLLYLNHGK GDGEWVPNKY GGHENLEAID FIKTLNKVIF
360 370 380 390 400
EYFPNPLMIA EESSAWPLVT YPAHLGGLGF NYKWNMGWMN DTLEYMEMDS
410 420 430 440 450
IYRKYHHNLL TFSFMYMFSE NFVLPLSHDE VVHGKKSLLD KMPGDYWQKF
460 470 480 490 500
ANLRTLYGYM MGHPGKKLLF MGGEFGQFIE WNYKQGLDWL LLDYDMHKKL
510 520 530 540 550
QNYVRELNYF YRQEKTLWEG DHEDGGFEWI DPHDSEQSII TFMRKNKDGS
560 570 580 590 600
DYTIIVCNFT PEVRHNYRIG VPEFKEYKEV FNSDLEKFGG SGQKNSTIIQ
610 620 630
PSEQPWHNRP YSIEITIPPL ATIFFKPLNQ
Length:630
Mass (Da):74,401
Last modified:March 3, 2009 - v1
Checksum:iC07E9BEBE09C7775
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001098 Genomic DNA. Translation: ACL69450.1.
RefSeqiWP_012635638.1. NC_011899.1.
YP_002508445.1. NC_011899.1.

Genome annotation databases

EnsemblBacteriaiACL69450; ACL69450; Hore_06930.
GeneIDi7312928.
KEGGihor:Hore_06930.
PATRICi22100738. VBIHalOre56292_0744.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001098 Genomic DNA. Translation: ACL69450.1.
RefSeqiWP_012635638.1. NC_011899.1.
YP_002508445.1. NC_011899.1.

3D structure databases

ProteinModelPortaliB8CVY1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi373903.Hore_06930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL69450; ACL69450; Hore_06930.
GeneIDi7312928.
KEGGihor:Hore_06930.
PATRICi22100738. VBIHalOre56292_0744.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciHORE373903:GHB1-729-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii."
    Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H., Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.
    PLoS ONE 4:E4192-E4192(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H 168 / OCM 544 / DSM 9562.

Entry informationi

Entry nameiGLGB_HALOH
AccessioniPrimary (citable) accession number: B8CVY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: January 7, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.