B8CUW9 (PLSB_SHEPW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	swp_4820

Organism

Shewanella piezotolerans (strain WP3 / JCM 13877) [Complete proteome] [HAMAP]

Taxonomic identifier

225849 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000123097
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B8CUW9 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 361887D638795347	FASTA	807	91,556
	10 20 30 40 50 60 MSKKDSLWFK SLRWIQKKLV HTIVVPHDPF DDLNLDPSKP LVYVMKTESV SDIAALSEMT 70 80 90 100 110 120 GTLGLPSPYD DLEVNGVSAP RVVCLEGSKP LFGERESAEQ YLHCFKRLLS VHKQNAELDI 130 140 150 160 170 180 QLVPVSLYWG RTPGKEDDTM RAAVLERQNP TWLRKCLMIL FLGRHNFVQF SNAVSLRYMA 190 200 210 220 230 240 DEHGTDKRIA QKLARVARVH FQRQRKVMTG PQLPKRQALF HALLKSDSIT KAIKEEAASK 250 260 270 280 290 300 KISETEARAK AMEYLDEVAA DYSDSLVRIA ERFLTWLWNK LYKGINIKGA EQIRQLHHDG 310 320 330 340 350 360 HEIVYVPCHR SHMDYLLISY ILYYQGMVPP HIAAGINLNF WPAGPMFRRG GAFFIRRSFR 370 380 390 400 410 420 GNKLYTAVFR EYLDQLFTKG YSVEYFTEGG RSRTGRLLAP KTGMIAMTLN SVLRGVERPV 430 440 450 460 470 480 TLVPVYLGYD HVMEVATYHK ELSGKKKKKE SVWQVFGAIR KLGNFGQGYV NFGKPITLHN 490 500 510 520 530 540 FLNDEVPNWR EDIAQDPEQK PTWLTPAVNT LASQVMTNIN DAAAVSSVTL TSLVLLASEQ 550 560 570 580 590 600 NALERSQLEK QLDLYLTLLK ELPYTEYTSV PEGSGKDLVS QGLELKKVQI ESDALGDIIS 610 620 630 640 650 660 IDDSIAITMT YYRNNIIHLL VVPSLIAACL LRKERCTRDD VVCVVNDFYP LLQAELFMGI 670 680 690 700 710 720 EDPKVYADSI LDIFVAQGLV IENGHFEVAD DKVNQLLLLS GTIDETMQRY AILFNLLAVK 730 740 750 760 770 780 PNMERSELER DSHRLAQRLG ALHGITAPEF YDKKLYATLS VKLKELGHLT DSEGCSDIKR 790 800 IKDRANLLLR SSVRQTIVDS VNAEQKD

References

[1]

"Environmental adaptation: genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3." Wang F., Wang J., Jian H., Zhang B., Li S., Wang F., Zeng X., Gao L., Bartlett D.H., Yu J., Hu S., Xiao X. PLoS ONE 3:E1937-E1937(2008) [PubMed: 18398463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WP3 / JCM 13877.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000472 Genomic DNA. Translation: ACJ31445.1.
RefSeq	YP_002314032.1. NC_011566.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8CUW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7035730.
GenomeReviews	Gene locus swp_4820 in contig CP000472_GR.
KEGG	swp:swp_4820.
PATRIC	23547871. VBIShePie65056_4431.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_SHEPW

Accession

Primary (citable) accession number: B8CUW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program